Effect of omega-3 fatty acid ethyl esters on the oxylipin composition of lipoproteins in hypertriglyceridemic, statin-treated subjects

John W. Newman, Theresa L. Pedersen, Verdayne R. Brandenburg, William Harris, Gregory C. Shearer

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: Oxylipins mediate inflammation, vascular tension, and more. Their presence in lipoproteins could explain why lipoproteins mediate nearly identical activities.

Methods: To determine how oxylipins are distributed in the lipoproteins of hypertriglyceridemic subjects, and whether omega-3 fatty acids alter them in a manner consistent with improved cardiovascular health, we recruited 15 dyslipidemic subjects whose levels of low density lipoprotein cholesterol (LDL-C) were at goal but who remained hypertriglyceridemic (200-499 mg/dL). They were treated them with the indicated dose of 4 g/d omega-3 acid ethyl esters (P-OM3) for 8 weeks. Measured oxylipins included mid-chain alcohols (HETEs, HEPEs and HDoHEs), ketones (KETEs), epoxides (as EpETrEs, EpETEs, and EpDPEs).

Results: At baseline, arachidonate-oxylipins (HETEs, KETEs, and EpETrEs) were most abundant in plasma with the greatest fraction of total abundance (mean |95% CI|) being carried in high density lipoproteins (HDL); 42% |31, 57| followed by very low density lipoproteins (VLDL); 27% |20, 36|; and LDL 21% |16, 28|. EPA- and DHA-derived oxylipins constituted less than 11% of total. HDL carried alcohols and epoxides but VLDL was also rich in ketones. Treatment decreased AA-derived oxylipins across lipoprotein classes (223% |233, 212|, p = 0.0003), and expanded EPA2(322% |241, 422|, p

Conclusions: Each lipoprotein class carries a unique oxylipin complement. P-OM3 treatment alters the oxylipin content of all classes, reducing pro-inflammatory and increasing anti-inflammatory species, consistent with the improved inflammatory and vascular status associated with the treatment.

Original languageEnglish (US)
Article number0111471
JournalPLoS One
Volume9
Issue number11
DOIs
StatePublished - Nov 13 2014
Externally publishedYes

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Oxylipins
oxylipins
Hydroxymethylglutaryl-CoA Reductase Inhibitors
Omega-3 Fatty Acids
lipoproteins
omega-3 fatty acids
Lipoproteins
Esters
esters
Chemical analysis
ketones
Ketones
Hydroxyeicosatetraenoic Acids
VLDL Lipoproteins
very low density lipoprotein
high density lipoprotein
Epoxy Compounds
HDL Lipoproteins
epoxides
blood vessels

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Effect of omega-3 fatty acid ethyl esters on the oxylipin composition of lipoproteins in hypertriglyceridemic, statin-treated subjects. / Newman, John W.; Pedersen, Theresa L.; Brandenburg, Verdayne R.; Harris, William; Shearer, Gregory C.

In: PLoS One, Vol. 9, No. 11, 0111471, 13.11.2014.

Research output: Contribution to journalArticle

Newman, John W. ; Pedersen, Theresa L. ; Brandenburg, Verdayne R. ; Harris, William ; Shearer, Gregory C. / Effect of omega-3 fatty acid ethyl esters on the oxylipin composition of lipoproteins in hypertriglyceridemic, statin-treated subjects. In: PLoS One. 2014 ; Vol. 9, No. 11.
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