Jonathan Pruneda

Assistant Professor

  • 1392 Citations
  • 16 h-Index
1980 …2019

Research output per year

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Research Output

2019

Evaluating enzyme activities and structures of DUBs

Pruneda, J. N. & Komander, D., 2019, Methods in Enzymology. Academic Press Inc., p. 321-341 21 p. (Methods in Enzymology; vol. 618).

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Insights into ubiquitin chain architecture using Ub-clipping

Swatek, K. N., Usher, J. L., Kueck, A. F., Gladkova, C., Mevissen, T. E. T., Pruneda, J. N., Skern, T. & Komander, D., Aug 22 2019, In : Nature. 572, 7770, p. 533-537 5 p.

Research output: Contribution to journalArticle

17 Scopus citations

Legionella dismantles linear ubiquitin

Damgaard, R. B. & Pruneda, J. N., Aug 1 2019, In : Nature Microbiology. 4, 8, p. 1244-1245 2 p.

Research output: Contribution to journalArticle

2018

A Chlamydia effector combining deubiquitination and acetylation activities induces Golgi fragmentation

Pruneda, J., Bastidas, R. J., Bertsoulaki, E., Swatek, K. N., Santhanam, B., Clague, M. J., Valdivia, R. H., Urbé, S. & Komander, D., Dec 1 2018, In : Nature Microbiology. 3, 12, p. 1377-1384 8 p.

Research output: Contribution to journalLetter

13 Scopus citations

Irreversible inactivation of ISG15 by a viral leader protease enables alternative infection detection strategies

Swatek, K. N., Aumayr, M., Pruneda, J. N., Visser, L. J., Berryman, S., Kueck, A. F., Geurink, P. P., Ovaa, H., van Kuppeveld, F. J. M., Tuthill, T. J., Skern, T. & Komander, D., Mar 6 2018, In : Proceedings of the National Academy of Sciences of the United States of America. 115, 10, p. 2371-2376 6 p.

Research output: Contribution to journalArticle

Open Access
13 Scopus citations
2017

An invisible ubiquitin conformation is required for efficient phosphorylation by PINK1

Gladkova, C., Schubert, A. F., Wagstaff, J. L., Pruneda, J. N., Freund, S. M. V. & Komander, D., Dec 15 2017, In : EMBO Journal. 36, 24, p. 3555-3572 18 p.

Research output: Contribution to journalArticle

Open Access
25 Scopus citations

Tuning BRCA1 and BARD1 activity to investigate RING ubiquitin ligase mechanisms

Stewart, M. D., Duncan, E. D., Coronado, E., DaRosa, P. A., Pruneda, J., Brzovic, P. S. & Klevit, R. E., Mar 1 2017, In : Protein Science. 26, 3, p. 475-483 9 p.

Research output: Contribution to journalArticle

Open Access
8 Scopus citations
2016

A cascading activity-based probe sequentially targets E1-E2-E3 ubiquitin enzymes

Mulder, M. P. C., Witting, K., Berlin, I., Pruneda, J., Wu, K. P., Chang, J. G., Merkx, R., Bialas, J., Groettrup, M., Vertegaal, A. C. O., Schulman, B. A., Komander, D., Neefjes, J., El Oualid, F. & Ovaa, H., Jul 1 2016, In : Nature Chemical Biology. 12, 7, p. 523-530 8 p.

Research output: Contribution to journalArticle

50 Scopus citations

Development of Diubiquitin-Based FRET Probes to Quantify Ubiquitin Linkage Specificity of Deubiquitinating Enzymes

Geurink, P. P., Van Tol, B. D. M., Van Dalen, D., Brundel, P. J. G., Mevissen, T. E. T., Pruneda, J., Elliott, P. R., Van Tilburg, G. B. A., Komander, D. & Ovaa, H., May 3 2016, In : ChemBioChem. 17, 9, p. 816-820 5 p.

Research output: Contribution to journalArticle

Open Access
28 Scopus citations

The Molecular Basis for Ubiquitin and Ubiquitin-like Specificities in Bacterial Effector Proteases

Pruneda, J., Durkin, C. H., Geurink, P. P., Ovaa, H., Santhanam, B., Holden, D. W. & Komander, D., Jul 21 2016, In : Molecular Cell. 63, 2, p. 261-276 16 p.

Research output: Contribution to journalArticle

Open Access
52 Scopus citations

The Salmonella effector SpvD is a cysteine hydrolase with a serovar-specific polymorphism influencing catalytic activity, suppression of immune responses, and bacterial virulence

Grabe, G. J., Zhang, Y., Przydacz, M., Rolhion, N., Yang, Y., Pruneda, J. N., Komander, D., Holden, D. W. & Hare, S. A., Dec 9 2016, In : Journal of Biological Chemistry. 291, 50, p. 25853-25863 11 p.

Research output: Contribution to journalArticle

Open Access
14 Scopus citations
2015

Assembly and specific recognition of K29- and K33-linked polyubiquitin

Michel, M. A., Elliott, P. R., Swatek, K. N., Simicek, M., Pruneda, J., Wagstaff, J. L., Freund, S. M. V. & Komander, D., Apr 2 2015, In : Molecular Cell. 58, 1, p. 95-109 15 p.

Research output: Contribution to journalArticle

Open Access
90 Scopus citations

Ubiquitin Ser65 phosphorylation affects ubiquitin structure, chain assembly and hydrolysis

Wauer, T., Swatek, K. N., Wagstaff, J. L., Gladkova, C., Pruneda, J. N., Michel, M. A., Gersch, M., Johnson, C. M., Freund, S. M. V. & Komander, D., Feb 3 2015, In : EMBO Journal. 34, 3, p. 307-325 19 p.

Research output: Contribution to journalArticle

Open Access
141 Scopus citations
2014

Allosteric activation of the RNF146 ubiquitin ligase by a poly(ADP-ribosyl)ation signal

DaRosa, P. A., Wang, Z., Jiang, X., Pruneda, J. N., Cong, F., Klevit, R. E. & Xu, W., Oct 19 2014, In : Nature. 517, 7533

Research output: Contribution to journalArticle

88 Scopus citations

E2∼Ub conjugates regulate the kinase activity of Shigella effector OspG during pathogenesis

Pruneda, J. N., Smith, F. D., Daurie, A., Swaney, D. L., Villén, J., Scott, J. D., Stadnyk, A. W., Le Trong, I., Stenkamp, R. E., Klevit, R. E., Rohde, J. R. & Brzovic, P. S., Mar 3 2014, In : EMBO Journal. 33, 5, p. 437-449 13 p.

Research output: Contribution to journalArticle

Open Access
39 Scopus citations

RING-type E3 ligases: Master manipulators of E2 ubiquitin-conjugating enzymes and ubiquitination

Metzger, M. B., Pruneda, J. N., Klevit, R. E. & Weissman, A. M., Jan 1 2014, In : Biochimica et Biophysica Acta - Molecular Cell Research. 1843, 1, p. 47-60 14 p.

Research output: Contribution to journalReview article

Open Access
248 Scopus citations
2013

Activity-enhancing mutations in an E3 ubiquitin ligase identified by high-throughput mutagenesis

Starita, L. M., Pruneda, J. N., Lo, R. S., Fowler, D. M., Kim, H. J., Hiatt, J. B., Shendure, J., Brzovic, P. S., Fields, S. & Klevit, R. E., Apr 2 2013, In : Proceedings of the National Academy of Sciences of the United States of America. 110, 14, p. E1263-E1272

Research output: Contribution to journalArticle

Open Access
70 Scopus citations
2012

OTUB1 Co-opts Lys48-Linked Ubiquitin Recognition to Suppress E2 Enzyme Function

Juang, Y. C., Landry, M. C., Sanches, M., Vittal, V., Leung, C. C. Y., Ceccarelli, D. F., Mateo, A. R. F., Pruneda, J. N., Mao, D. Y. L., Szilard, R. K., Orlicky, S., Munro, M., Brzovic, P. S., Klevit, R. E., Sicheri, F. & Durocher, D., Feb 10 2012, In : Molecular Cell. 45, 3, p. 384-397 14 p.

Research output: Contribution to journalArticle

Open Access
108 Scopus citations

OTUB1 Co-opts Lys48-Linked Ubiquitin Recognition to Suppress E2 Enzyme Function

Juang, Y. C., Landry, M. C., Sanches, M., Vittal, V., Leung, C., Ceccarelli, D. F., Mateo, A. R. F., Pruneda, J. N., Mao, D., Szilard, R. K., Orlicky, S., Munro, M., Brzovic, P. S., Klevit, R. E., Sicheri, F. & Durocher, D., May 25 2012, In : Molecular Cell. 46, 4, 1 p.

Research output: Contribution to journalComment/debate

Open Access
2 Scopus citations

Structural insights into the conformation and oligomerization of e2∼ubiquitin conjugates

Page, R. C., Pruneda, J. N., Amick, J., Klevit, R. E. & Misra, S., May 22 2012, In : Biochemistry. 51, 20, p. 4175-4187 13 p.

Research output: Contribution to journalArticle

45 Scopus citations

Structure of an E3:E2∼Ub Complex Reveals an Allosteric Mechanism Shared among RING/U-box Ligases

Pruneda, J. N., Littlefield, P. J., Soss, S. E., Nordquist, K. A., Chazin, W. J., Brzovic, P. S. & Klevit, R. E., Sep 28 2012, In : Molecular Cell. 47, 6, p. 933-942 10 p.

Research output: Contribution to journalArticle

Open Access
180 Scopus citations
2011

Structural and functional studies on the interaction of gspc and gspd in the type ii secretion system

Korotkov, K. V., Johnson, T. L., Jobling, M. G., Pruneda, J., Pardon, E., Héroux, A., Turley, S., Steyaert, J., Holmes, R. K., Sandkvist, M. & Hol, W. G. J., Sep 2011, In : PLoS pathogens. 7, 9, e1002228.

Research output: Contribution to journalArticle

Open Access
59 Scopus citations

Ubiquitin in motion: Structural studies of the ubiquitin-conjugating enzyme∼ubiquitin conjugate

Pruneda, J. N., Stoll, K. E., Bolton, L. J., Brzovic, P. S. & Klevit, R. E., Mar 15 2011, In : Biochemistry. 50, 10, p. 1624-1633 10 p.

Research output: Contribution to journalArticle

85 Scopus citations
2008

Semi-field evaluation of a granulovirus and Bacillus thuringiensis ssp. kurstaki for season-long control of the potato tuber moth, Phthorimaea operculella

Arthurs, S. P., Lacey, L. A., Pruneda, J. N. & Rondon, S. I., Dec 1 2008, In : Entomologia Experimentalis et Applicata. 129, 3, p. 276-285 10 p.

Research output: Contribution to journalArticle

16 Scopus citations