Yersinia enterocolitica YopT and clostridium difficile toxin B induce expression of GILZ in epithelial cells

Martin Köberle, David Göppel, Tanja Grandl, Peer Gaentzsch, Birgit Manncke, Susanne Berchtold, Steffen Müller, Bernhard Lüscher, Marie-Liesse Labat, Marc Pallardy, Isabel Sorg, Simon Langer, Holger Barth, Robert Zumbihl, Ingo B. Autenrieth, Erwin Bohn

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Glucocorticoid induced-leucine zipper (GILZ) has been shown to be induced in cells by different stimuli such as glucocorticoids, IL-10 or deprivation of IL-2. GILZ has anti-inflammatory properties and may be involved in signalling modulating apoptosis. Herein we demonstrate that wildtype Yersinia enterocolitica which carry the pYV plasmid upregulated GILZ mRNA levels and protein expression in epithelial cells. Infection of HeLa cells with different Yersinia mutant strains revealed that the protease activity of YopT, which cleaves the membrane-bound form of Rho GTPases was sufficient to induce GILZ expression. Similarly, Clostridium difficile toxin B, another bacterial inhibitor of Rho GTPases induced GILZ expression. YopT and toxin B both increased transcriptional activity of the GILZ promoter in HeLa cells. GILZ expression could not be linked to the inactivation of an individual Rho GTPase by these toxins. However, forced expression of RhoA and RhoB decreased basal GILZ promoter activity. Furthermore, MAPK activation proved necessary for profound GILZ induction by toxin B. Promoter studies and gel shift analyses defined binding of upstream stimulatory factor (USF) 1 and 2 to a canonical c-Myc binding site (E-box) in the GILZ promoter as a crucial step of its trans-activation. In addition we could show that USF-1 and USF-2 are essential for basal as well as toxin B induced GILZ expression. These findings define a novel way of GILZ promoter trans-activation mediated by bacterial toxins and differentiate it from those mediated by dexamethasone or deprivation of IL-2.

Original languageEnglish (US)
Article numbere40730
JournalPloS one
Volume7
Issue number7
DOIs
StatePublished - Jul 9 2012
Externally publishedYes

Fingerprint

Clostridium difficile
Leucine Zippers
leucine zipper
Yersinia enterocolitica
glucocorticoids
Glucocorticoids
epithelial cells
toxins
Epithelial Cells
Upstream Stimulatory Factors
rho GTP-Binding Proteins
promoter regions
guanosinetriphosphatase
Chemical activation
transcriptional activation
interleukin-2
HeLa Cells
Clostridium difficile toxB protein
Interleukin-2
bacterial toxins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Köberle, M., Göppel, D., Grandl, T., Gaentzsch, P., Manncke, B., Berchtold, S., ... Bohn, E. (2012). Yersinia enterocolitica YopT and clostridium difficile toxin B induce expression of GILZ in epithelial cells. PloS one, 7(7), [e40730]. https://doi.org/10.1371/journal.pone.0040730

Yersinia enterocolitica YopT and clostridium difficile toxin B induce expression of GILZ in epithelial cells. / Köberle, Martin; Göppel, David; Grandl, Tanja; Gaentzsch, Peer; Manncke, Birgit; Berchtold, Susanne; Müller, Steffen; Lüscher, Bernhard; Labat, Marie-Liesse; Pallardy, Marc; Sorg, Isabel; Langer, Simon; Barth, Holger; Zumbihl, Robert; Autenrieth, Ingo B.; Bohn, Erwin.

In: PloS one, Vol. 7, No. 7, e40730, 09.07.2012.

Research output: Contribution to journalArticle

Köberle, M, Göppel, D, Grandl, T, Gaentzsch, P, Manncke, B, Berchtold, S, Müller, S, Lüscher, B, Labat, M-L, Pallardy, M, Sorg, I, Langer, S, Barth, H, Zumbihl, R, Autenrieth, IB & Bohn, E 2012, 'Yersinia enterocolitica YopT and clostridium difficile toxin B induce expression of GILZ in epithelial cells', PloS one, vol. 7, no. 7, e40730. https://doi.org/10.1371/journal.pone.0040730
Köberle, Martin ; Göppel, David ; Grandl, Tanja ; Gaentzsch, Peer ; Manncke, Birgit ; Berchtold, Susanne ; Müller, Steffen ; Lüscher, Bernhard ; Labat, Marie-Liesse ; Pallardy, Marc ; Sorg, Isabel ; Langer, Simon ; Barth, Holger ; Zumbihl, Robert ; Autenrieth, Ingo B. ; Bohn, Erwin. / Yersinia enterocolitica YopT and clostridium difficile toxin B induce expression of GILZ in epithelial cells. In: PloS one. 2012 ; Vol. 7, No. 7.
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abstract = "Glucocorticoid induced-leucine zipper (GILZ) has been shown to be induced in cells by different stimuli such as glucocorticoids, IL-10 or deprivation of IL-2. GILZ has anti-inflammatory properties and may be involved in signalling modulating apoptosis. Herein we demonstrate that wildtype Yersinia enterocolitica which carry the pYV plasmid upregulated GILZ mRNA levels and protein expression in epithelial cells. Infection of HeLa cells with different Yersinia mutant strains revealed that the protease activity of YopT, which cleaves the membrane-bound form of Rho GTPases was sufficient to induce GILZ expression. Similarly, Clostridium difficile toxin B, another bacterial inhibitor of Rho GTPases induced GILZ expression. YopT and toxin B both increased transcriptional activity of the GILZ promoter in HeLa cells. GILZ expression could not be linked to the inactivation of an individual Rho GTPase by these toxins. However, forced expression of RhoA and RhoB decreased basal GILZ promoter activity. Furthermore, MAPK activation proved necessary for profound GILZ induction by toxin B. Promoter studies and gel shift analyses defined binding of upstream stimulatory factor (USF) 1 and 2 to a canonical c-Myc binding site (E-box) in the GILZ promoter as a crucial step of its trans-activation. In addition we could show that USF-1 and USF-2 are essential for basal as well as toxin B induced GILZ expression. These findings define a novel way of GILZ promoter trans-activation mediated by bacterial toxins and differentiate it from those mediated by dexamethasone or deprivation of IL-2.",
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