X-chromosome-wide profiling of MSL-1 distribution and dosage compensation in Drosophila

Gaëlle Legube, Shannon McWeeney, Martin J. Lercher, Asifa Akhtar

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

In Drosophila, dosage compensation is achieved by a twofold up-regulation of the male X-linked genes and requires the association of the male-specific lethal complex (MSL) on the X chromosome. How the MSL complex is targeted to X-linked genes and whether its recruitment at a local level is necessary and sufficient to ensure dosage compensation remain poorly understood. Here we report the MSL-1-binding profile along the male X chromosome in embryos and male salivary glands isolated from third instar larvae using chromatin immunoprecipitation (ChIP) coupled with DNA microarray (ChIP-chip). This analysis has revealed that majority of the MSL-1 targets are primarily expressed during early embryogenesis and many target genes possess DNA replication element factor (DREF)-binding sites in their promoters. In addition, we show that MSL-1 distribution remains stable across development and that binding of MSL-1 on X-chromosomal genes does not correlate with transcription in male salivary glands. These results show that transcription per se on the X chromosome cannot be the sole signal for MSL-1 recruitment. Furthermore, genome-wide analysis of the dosage-compensated status of X-linked genes in male and female shows that most of the X chromosome remains compensated without direct MSL-1 binding near the gene. Our results, therefore, provide a comprehensive overview of MSL-1 binding and dosage-compensated status of X-linked genes and suggest a more global effect of MSL complex on X-chromosome regulation.

Original languageEnglish (US)
Pages (from-to)871-883
Number of pages13
JournalGenes and Development
Volume20
Issue number7
DOIs
StatePublished - Apr 1 2006

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X Chromosome
Drosophila
X-Linked Genes
Chromatin Immunoprecipitation
Salivary Glands
Genes
Oligonucleotide Array Sequence Analysis
DNA Replication
Embryonic Development
Larva

Keywords

  • Chromatin
  • Dosage compensation
  • MSL
  • Transcription
  • X chromosome

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

X-chromosome-wide profiling of MSL-1 distribution and dosage compensation in Drosophila. / Legube, Gaëlle; McWeeney, Shannon; Lercher, Martin J.; Akhtar, Asifa.

In: Genes and Development, Vol. 20, No. 7, 01.04.2006, p. 871-883.

Research output: Contribution to journalArticle

Legube, Gaëlle ; McWeeney, Shannon ; Lercher, Martin J. ; Akhtar, Asifa. / X-chromosome-wide profiling of MSL-1 distribution and dosage compensation in Drosophila. In: Genes and Development. 2006 ; Vol. 20, No. 7. pp. 871-883.
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