TY - JOUR
T1 - Vitamin D metabolites and the gut microbiome in older men
AU - Thomas, Robert L.
AU - Jiang, Lingjing
AU - Adams, John S.
AU - Xu, Zhenjiang Zech
AU - Shen, Jian
AU - Janssen, Stefan
AU - Ackermann, Gail
AU - Vanderschueren, Dirk
AU - Pauwels, Steven
AU - Knight, Rob
AU - Orwoll, Eric S.
AU - Kado, Deborah M.
N1 - Funding Information:
We thank Loki Natarajan, PhD for suggestions on statistical analysis. We are also grateful to the MrOS study participants for their continued dedication to this study and to the other principal investigators and staff of the MrOS study centers: (1) James Shikany, University of Alabama, Birmingham, AL, USA; (2) Kristine Ensrud, University of Minnesota, Minneapolis, MN, USA; (3) Jane Cauley, University of Pittsburgh, Pittsburgh, PA, USA; and (4) Marcia Stefanick, Stanford University, Palo Alto, CA, USA. This work was supported by the National Institutes of Health (grant numbers U01 AG027810, U01 AG042124, U01 AG042139, U01 AG042140, U01 AG042143, U01 AG042145, U01 AG042168, U01 AR066160, and UL1 TR000128). We would also like to thankfully acknowledge the Dr. Ruth Covell Fund for Geriatric Research and Education for covering the publication costs.
Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12
Y1 - 2020/12
N2 - The vitamin D receptor is highly expressed in the gastrointestinal tract where it transacts gene expression. With current limited understanding of the interactions between the gut microbiome and vitamin D, we conduct a cross-sectional analysis of 567 older men quantifying serum vitamin D metabolites using LC-MSMS and defining stool sub-Operational Taxonomic Units from16S ribosomal RNA gene sequencing data. Faith’s Phylogenetic Diversity and non-redundant covariate analyses reveal that the serum 1,25(OH)2D level explains 5% of variance in α-diversity. In β-diversity analyses using unweighted UniFrac, 1,25(OH)2D is the strongest factor assessed, explaining 2% of variance. Random forest analyses identify 12 taxa, 11 in the phylum Firmicutes, eight of which are positively associated with either 1,25(OH)2D and/or the hormone-to-prohormone [1,25(OH)2D/25(OH)D] “activation ratio.” Men with higher levels of 1,25(OH)2D and higher activation ratios, but not 25(OH)D itself, are more likely to possess butyrate producing bacteria that are associated with better gut microbial health.
AB - The vitamin D receptor is highly expressed in the gastrointestinal tract where it transacts gene expression. With current limited understanding of the interactions between the gut microbiome and vitamin D, we conduct a cross-sectional analysis of 567 older men quantifying serum vitamin D metabolites using LC-MSMS and defining stool sub-Operational Taxonomic Units from16S ribosomal RNA gene sequencing data. Faith’s Phylogenetic Diversity and non-redundant covariate analyses reveal that the serum 1,25(OH)2D level explains 5% of variance in α-diversity. In β-diversity analyses using unweighted UniFrac, 1,25(OH)2D is the strongest factor assessed, explaining 2% of variance. Random forest analyses identify 12 taxa, 11 in the phylum Firmicutes, eight of which are positively associated with either 1,25(OH)2D and/or the hormone-to-prohormone [1,25(OH)2D/25(OH)D] “activation ratio.” Men with higher levels of 1,25(OH)2D and higher activation ratios, but not 25(OH)D itself, are more likely to possess butyrate producing bacteria that are associated with better gut microbial health.
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U2 - 10.1038/s41467-020-19793-8
DO - 10.1038/s41467-020-19793-8
M3 - Article
C2 - 33244003
AN - SCOPUS:85096667673
SN - 2041-1723
VL - 11
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 5997
ER -