Vitamin D does not modulate NF-κB activity in Jurkat T cells

Amde Selassie Shifera, Deborah Leong, John A. Hardin

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The active form of vitamin D, 1α,25-dihydroxyvitamin D3 [1α,25(OH)2D3], has been reported to influence the functioning of the immune system by targeting the activities of cellular signaling pathways, in addition to its direct genomic effects. One of the signaling pathways reported to be targeted by vitamin D is the NF-κB pathway, which is highly active in most immune cell types, including T cells. However, the effects of vitamin D on the NF-κB pathway in T cells are not fully understood. Therefore, we examined the effects of 1α,25(OH)2D3 on the NF-κB pathway in the Jurkat cell line, a human T cell line that constitutively expresses endogenous vitamin D receptor. We found that 1α,25(OH)2D3 does not inhibit the induction of IκBα degradation and the expression of an NF-κB-dependent reporter gene in Jurkat cells following treatment with PMA/ionomycin. Also, 1α,25(OH)2D3 did not suppress the activation of NF-κB by TNFα or PHA. Furthermore, we demonstrate that 1α,25(OH)2D3 does not block the induction of CD69, which is an NF-κB target gene and an early T cell activation marker. Therefore, we conclude that vitamin D does not modulate the activity of the NF-κB pathway in Jurkat cells.

Original languageEnglish (US)
Pages (from-to)151-158
Number of pages8
JournalImmunology Letters
Volume131
Issue number2
DOIs
StatePublished - Jul 2010
Externally publishedYes

Fingerprint

Jurkat Cells
Vitamin D
T-Lymphocytes
Cell Line
Calcitriol Receptors
Ionomycin
Calcitriol
Reporter Genes
Immune System
Genes

Keywords

  • CD69
  • Jurkat cells
  • NF-κB
  • T cells
  • Vitamin D

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Medicine(all)

Cite this

Vitamin D does not modulate NF-κB activity in Jurkat T cells. / Shifera, Amde Selassie; Leong, Deborah; Hardin, John A.

In: Immunology Letters, Vol. 131, No. 2, 07.2010, p. 151-158.

Research output: Contribution to journalArticle

Shifera, Amde Selassie ; Leong, Deborah ; Hardin, John A. / Vitamin D does not modulate NF-κB activity in Jurkat T cells. In: Immunology Letters. 2010 ; Vol. 131, No. 2. pp. 151-158.
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