VioC is a non-heme iron, α-ketoglutarate-dependent oxygenase that catalyzes the formation of 3S-hydroxy-L-arginine during viomycin biosynthesis

Xihou Yin, Mark Zabriskie

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

TB or no TB? Viomycin is a peptide antibiotic possessing several nonproteinogenic amino acids and is an effective agent in the treatment of multidrug resistant tuberculosis. The vioC gene from the recently cloned viomycin biosynthesis gene cluster was expressed in E. coli, and the corresponding protein was shown to catalyze the conversion of L-arginine to 3S-hydroxy-L-arginine in the presence of Fe2+ and α-ketoglutarate. VioC is the first example of an arginine β-hydroxylase, and the product is a putative precursor to the unusual amino acid capreomycidine.

Original languageEnglish (US)
Pages (from-to)1274-1277
Number of pages4
JournalChemBioChem
Volume5
Issue number9
DOIs
StatePublished - Sep 6 2004
Externally publishedYes

Fingerprint

Viomycin
Oxygenases
Biosynthesis
Arginine
Iron
Genes
Amino Acids
Multidrug-Resistant Tuberculosis
Escherichia coli Proteins
Multigene Family
Mixed Function Oxygenases
Anti-Bacterial Agents
Peptides

Keywords

  • Antibiotics
  • Arginine
  • Biosynthesis
  • Iron
  • Proteins
  • Viomycin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Organic Chemistry
  • Drug Discovery

Cite this

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abstract = "TB or no TB? Viomycin is a peptide antibiotic possessing several nonproteinogenic amino acids and is an effective agent in the treatment of multidrug resistant tuberculosis. The vioC gene from the recently cloned viomycin biosynthesis gene cluster was expressed in E. coli, and the corresponding protein was shown to catalyze the conversion of L-arginine to 3S-hydroxy-L-arginine in the presence of Fe2+ and α-ketoglutarate. VioC is the first example of an arginine β-hydroxylase, and the product is a putative precursor to the unusual amino acid capreomycidine.",
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T1 - VioC is a non-heme iron, α-ketoglutarate-dependent oxygenase that catalyzes the formation of 3S-hydroxy-L-arginine during viomycin biosynthesis

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AU - Zabriskie, Mark

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Y1 - 2004/9/6

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AB - TB or no TB? Viomycin is a peptide antibiotic possessing several nonproteinogenic amino acids and is an effective agent in the treatment of multidrug resistant tuberculosis. The vioC gene from the recently cloned viomycin biosynthesis gene cluster was expressed in E. coli, and the corresponding protein was shown to catalyze the conversion of L-arginine to 3S-hydroxy-L-arginine in the presence of Fe2+ and α-ketoglutarate. VioC is the first example of an arginine β-hydroxylase, and the product is a putative precursor to the unusual amino acid capreomycidine.

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KW - Arginine

KW - Biosynthesis

KW - Iron

KW - Proteins

KW - Viomycin

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