Uveitis seroreactivity to candidate pANCA antigens: Mycobacterial HupB and histone H1(69-171)

Hallie Kassan; Offer Cohavy; James T. Rosenbaum; Jonathan Braun; Lynn K. Gordon

doi:10.1080/09273940490912452

Uveitis seroreactivity to candidate pANCA antigens: Mycobacterial HupB and histone H1(69-171)

Hallie Kassan, Offer Cohavy, James T. Rosenbaum, Jonathan Braun, Lynn K. Gordon

Ophthalmology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Purpose: Certain uveitis patients express the disease-marker antibody pANCA. Histone H1 (H1) and mycobacterial HupB (HupB) are recently identified candidate pANCA antigens. This study addresses the hypothesis that H1 and HupB are targets of disease-associated seroreactivity in pANCA+ uveitis. Methods: Sera from 293 uveitis patients were categorized for pANCA activity. H1(69-171) and HupB recombinant proteins were used as antigenic targets in ELISA studies. Selected sera were analyzed for reactivity by Western blot. Results: Seroreactivities to ANCA, H1(69-171), and HupB were frequently detected in uveitis patients. No significant relationship existed between the level of ANCA antibody and reactivity to either recombinant antigen. There was, however, a significant, positive correlation between H1(69-171) and HupB seroreactivities. Conclusions: Correlation between H1(69-171) and HupB reactivities suggests the presence of a shared dominant epitope, which is recognized by antibodies in a substantial number of uveitis patients. Seroreactivities to ANCA and HupB/H1 are independent immunologic markers that may identify biologically distinctive subsets of uveitis.

Original language	English (US)
Pages (from-to)	191-198
Number of pages	8
Journal	Ocular Immunology and Inflammation
Volume	13
Issue number	2-3
DOIs	https://doi.org/10.1080/09273940490912452
State	Published - Apr 2005

Keywords

Histone H1(69-171)
HupB
Inflammation
Uveitis
pANCA

ASJC Scopus subject areas

Immunology and Allergy
Ophthalmology

Access to Document

10.1080/09273940490912452

Cite this

@article{b1c6e8ad9da0431b9088b19d74927e0b,

title = "Uveitis seroreactivity to candidate pANCA antigens: Mycobacterial HupB and histone H1(69-171)",

abstract = "Purpose: Certain uveitis patients express the disease-marker antibody pANCA. Histone H1 (H1) and mycobacterial HupB (HupB) are recently identified candidate pANCA antigens. This study addresses the hypothesis that H1 and HupB are targets of disease-associated seroreactivity in pANCA+ uveitis. Methods: Sera from 293 uveitis patients were categorized for pANCA activity. H1(69-171) and HupB recombinant proteins were used as antigenic targets in ELISA studies. Selected sera were analyzed for reactivity by Western blot. Results: Seroreactivities to ANCA, H1(69-171), and HupB were frequently detected in uveitis patients. No significant relationship existed between the level of ANCA antibody and reactivity to either recombinant antigen. There was, however, a significant, positive correlation between H1(69-171) and HupB seroreactivities. Conclusions: Correlation between H1(69-171) and HupB reactivities suggests the presence of a shared dominant epitope, which is recognized by antibodies in a substantial number of uveitis patients. Seroreactivities to ANCA and HupB/H1 are independent immunologic markers that may identify biologically distinctive subsets of uveitis.",

keywords = "Histone H1(69-171), HupB, Inflammation, Uveitis, pANCA",

author = "Hallie Kassan and Offer Cohavy and Rosenbaum, {James T.} and Jonathan Braun and Gordon, {Lynn K.}",

note = "Funding Information: Acknowledgements: LKG is a James S. Adams Scholar of Research to Prevent Blindness. JTR is a Research to Prevent Blindness Senior Scholar. Funded in part by NIH EY00360 and EY13708.",

year = "2005",

month = apr,

doi = "10.1080/09273940490912452",

language = "English (US)",

volume = "13",

pages = "191--198",

journal = "Ocular Immunology and Inflammation",

issn = "0927-3948",

publisher = "Informa Healthcare",

number = "2-3",

}

TY - JOUR

T1 - Uveitis seroreactivity to candidate pANCA antigens

T2 - Mycobacterial HupB and histone H1(69-171)

AU - Kassan, Hallie

AU - Cohavy, Offer

AU - Rosenbaum, James T.

AU - Braun, Jonathan

AU - Gordon, Lynn K.

N1 - Funding Information: Acknowledgements: LKG is a James S. Adams Scholar of Research to Prevent Blindness. JTR is a Research to Prevent Blindness Senior Scholar. Funded in part by NIH EY00360 and EY13708.

PY - 2005/4

Y1 - 2005/4

N2 - Purpose: Certain uveitis patients express the disease-marker antibody pANCA. Histone H1 (H1) and mycobacterial HupB (HupB) are recently identified candidate pANCA antigens. This study addresses the hypothesis that H1 and HupB are targets of disease-associated seroreactivity in pANCA+ uveitis. Methods: Sera from 293 uveitis patients were categorized for pANCA activity. H1(69-171) and HupB recombinant proteins were used as antigenic targets in ELISA studies. Selected sera were analyzed for reactivity by Western blot. Results: Seroreactivities to ANCA, H1(69-171), and HupB were frequently detected in uveitis patients. No significant relationship existed between the level of ANCA antibody and reactivity to either recombinant antigen. There was, however, a significant, positive correlation between H1(69-171) and HupB seroreactivities. Conclusions: Correlation between H1(69-171) and HupB reactivities suggests the presence of a shared dominant epitope, which is recognized by antibodies in a substantial number of uveitis patients. Seroreactivities to ANCA and HupB/H1 are independent immunologic markers that may identify biologically distinctive subsets of uveitis.

AB - Purpose: Certain uveitis patients express the disease-marker antibody pANCA. Histone H1 (H1) and mycobacterial HupB (HupB) are recently identified candidate pANCA antigens. This study addresses the hypothesis that H1 and HupB are targets of disease-associated seroreactivity in pANCA+ uveitis. Methods: Sera from 293 uveitis patients were categorized for pANCA activity. H1(69-171) and HupB recombinant proteins were used as antigenic targets in ELISA studies. Selected sera were analyzed for reactivity by Western blot. Results: Seroreactivities to ANCA, H1(69-171), and HupB were frequently detected in uveitis patients. No significant relationship existed between the level of ANCA antibody and reactivity to either recombinant antigen. There was, however, a significant, positive correlation between H1(69-171) and HupB seroreactivities. Conclusions: Correlation between H1(69-171) and HupB reactivities suggests the presence of a shared dominant epitope, which is recognized by antibodies in a substantial number of uveitis patients. Seroreactivities to ANCA and HupB/H1 are independent immunologic markers that may identify biologically distinctive subsets of uveitis.

KW - Histone H1(69-171)

KW - HupB

KW - Inflammation

KW - Uveitis

KW - pANCA

UR - http://www.scopus.com/inward/record.url?scp=25144479610&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=25144479610&partnerID=8YFLogxK

U2 - 10.1080/09273940490912452

DO - 10.1080/09273940490912452

M3 - Article

C2 - 16019678

AN - SCOPUS:25144479610

SN - 0927-3948

VL - 13

SP - 191

EP - 198

JO - Ocular Immunology and Inflammation

JF - Ocular Immunology and Inflammation

IS - 2-3

ER -

Uveitis seroreactivity to candidate pANCA antigens: Mycobacterial HupB and histone H1(69-171)

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this