Uveitis seroreactivity to candidate pANCA antigens: Mycobacterial HupB and histone H1(69-171)

Hallie Kassan, Offer Cohavy, James T. Rosenbaum, Jonathan Braun, Lynn K. Gordon

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Purpose: Certain uveitis patients express the disease-marker antibody pANCA. Histone H1 (H1) and mycobacterial HupB (HupB) are recently identified candidate pANCA antigens. This study addresses the hypothesis that H1 and HupB are targets of disease-associated seroreactivity in pANCA+ uveitis. Methods: Sera from 293 uveitis patients were categorized for pANCA activity. H1(69-171) and HupB recombinant proteins were used as antigenic targets in ELISA studies. Selected sera were analyzed for reactivity by Western blot. Results: Seroreactivities to ANCA, H1(69-171), and HupB were frequently detected in uveitis patients. No significant relationship existed between the level of ANCA antibody and reactivity to either recombinant antigen. There was, however, a significant, positive correlation between H1(69-171) and HupB seroreactivities. Conclusions: Correlation between H1(69-171) and HupB reactivities suggests the presence of a shared dominant epitope, which is recognized by antibodies in a substantial number of uveitis patients. Seroreactivities to ANCA and HupB/H1 are independent immunologic markers that may identify biologically distinctive subsets of uveitis.

Original languageEnglish (US)
Pages (from-to)191-198
Number of pages8
JournalOcular Immunology and Inflammation
Volume13
Issue number2-3
DOIs
StatePublished - Apr 1 2005

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Keywords

  • Histone H1(69-171)
  • HupB
  • Inflammation
  • Uveitis
  • pANCA

ASJC Scopus subject areas

  • Immunology and Allergy
  • Ophthalmology

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