UUCAC- and Vera-dependent localization of VegT RNA in Xenopus oocytes

Sunjong Kwon, Tatiana Abramson, Trent P. Munro, Corinne M. John, Martin Köhrmann, Bruce Schnapp

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Localized mRNAs are directed to their destinations by "localization elements" (LEs) in their 3′UTRs. LEs harbor multiple, functionally redundant localization "signals." These signals are poorly defined, hence it is unclear whether the signals - and their cognate factors - are unique to each RNA or employed generally. Five "E2s" (UUCACs) in the 366 nt Vg1 LE (VgLE) direct this transcript to the vegetal pole of Xenopus oocytes via the binding of a protein - Vera/Vg1RBP/ZBP [1-3]. Here we show that a different vegetal RNA, VegT, employs the same signal and factor. Five E2s within a 440 nt subregion (VegT440) of the VegT 3′UTR predict its LE and are both necessary and sufficient (in the context of antisense VegT440) for directing localization. The E2s in VegT440 and VgLE function similarly to recruit Vera protein: (1) in both contexts, E2 nt substitutions partially (UU to AC) or completely (CA to UG) inhibit localization in accordance with the sequence selectivity of Vera protein for E2s; (2) VegT440 and VgLE crosscompete, in an E2-dependent manner, for localization and Vera binding; (3) injection of anti-Vera antibody into oocytes inhibits localization of both injected transcripts. These findings imply that general localization signals traffic diverse RNAs.

Original languageEnglish (US)
Pages (from-to)558-564
Number of pages7
JournalCurrent Biology
Volume12
Issue number7
DOIs
StatePublished - Apr 2 2002

Fingerprint

Xenopus
Oocytes
oocytes
RNA
Traffic signals
Proteins
Ports and harbors
traffic
binding proteins
Anti-Idiotypic Antibodies
Poles
Carrier Proteins
Substitution reactions
proteins
injection
Messenger RNA
Injections
antibodies
Antibodies

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

UUCAC- and Vera-dependent localization of VegT RNA in Xenopus oocytes. / Kwon, Sunjong; Abramson, Tatiana; Munro, Trent P.; John, Corinne M.; Köhrmann, Martin; Schnapp, Bruce.

In: Current Biology, Vol. 12, No. 7, 02.04.2002, p. 558-564.

Research output: Contribution to journalArticle

Kwon, Sunjong ; Abramson, Tatiana ; Munro, Trent P. ; John, Corinne M. ; Köhrmann, Martin ; Schnapp, Bruce. / UUCAC- and Vera-dependent localization of VegT RNA in Xenopus oocytes. In: Current Biology. 2002 ; Vol. 12, No. 7. pp. 558-564.
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