Uroplakin II outperforms uroplakin III in diagnostically challenging settings

Steven C. Smith, Sambit K. Mohanty, Lakshmi P. Kunju, Elena Chang, Fai Chung, Jason C. Carvalho, Gladel P. Paner, Donna E. Hansel, Daniel J. Luthringer, Mariza N. de Peralta-Ventrurina, Mahul B. Amin

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Aims: We performed a head-to-head comparison of an antibody against uroplakin III (UP3) and a new uroplakin II (UP2) antibody that remains untested in diagnostically challenging settings. Methods and results: We immunostained high-grade bladder neck carcinomas (n = 35), high-grade upper tract urothelial carcinomas (UC) and renal carcinomas (n = 85), metastases of UC (n = 30) and a multicancer tissue microarray (n = 88) for UP3 and UP2, and scored staining intensity and proportion. UP3 showed membranous plaque-like expression, while UP2 staining showed both membranous and cytoplasmic positivity. Significantly greater intensity (P = 0.003) and proportion (P = 0.03) of staining was noted for UP2 among bladder neck lesions, with UP2 staining showing greater sensitivity (63% versus 19%) and similar specificity (95% versus 100%) for UC over prostate carcinoma (P = 0.02). Among upper tract lesions, UP2 staining showed greater intensity and proportion than UP3 (both P < 0.001), including improved sensitivity (68% versus 23%) and equal specificity (both 100%) for UC (P = 0.006). Among UC metastases, UP2 staining showed greater intensity and proportion (both P < 0.001) with higher sensitivity (73% versus 37%, respectively, P = 0.001). Of 88 additional cases tested, no non-urothelial cases stained for either UP. Conclusions: The UP2 antibody outperforms the UP3 antibody, including in diagnostically challenging settings, and is a useful addition to the armamentarium of biomarkers for UC.

Original languageEnglish (US)
Pages (from-to)132-138
Number of pages7
JournalHistopathology
Volume65
Issue number1
DOIs
StatePublished - Jul 2014
Externally publishedYes

Keywords

  • Biomarkers
  • Immunohistochemistry
  • Uroplakin
  • Urothelial carcinoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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