Urea and NaCl differentially regulate FAK and RAFTK/PYK2 in mIMCD3 renal medullary cells

Zheng Zhang, Hava Avraham, David Cohen

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Two cytosolic tyrosine kinases, focal adhesion kinase (FAK) and the newly described FAK homolog, related adhesion focal tyrosine kinase (RAFTK, also called PYK2 and CAKβ), have been implicated in signaling to multiple mitogen-activated protein kinase (MAPK) pathways. Therefore, the ability of NaCl and urea to activate these kinases was investigated by in vitro kinase assay and anti-phosphotyrosine immunoblotting. RAFTK was promptly but only transiently activated by urea (within i min; 45%), whereas NaCl activated this kinase at 1, 5, 15, and 30 min of treatment (35-60%). In contrast, FAK exhibited only subtle regulation by the two solutes; however, the time course of induction was distinct for each solute. NaCl activated FAK at 1, 5, and 15 min (25-40%), whereas urea-inducible FAK activation (30%) was not evident until fully 15 min of treatment. At 5 min of treatment with increasing concentrations of solute, both urea and NaCl activated RAFTK in a dose- dependent and comparable fashion, culminating in an approximately twofold activation at 800 mosmol/kgH2O solute. Consistent with these data, solute treatment also enhanced tyrosine phosphorylation of RAFTK.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume275
Issue number3 44-3
StatePublished - Sep 1998

Fingerprint

Focal Adhesion Protein-Tyrosine Kinases
Urea
Kidney
Phosphotransferases
Focal Adhesion Kinase 1
Focal Adhesion Kinase 2
Phosphotyrosine
Mitogen-Activated Protein Kinases
Immunoblotting
Protein-Tyrosine Kinases
Tyrosine
Phosphorylation

Keywords

  • Focal adhesion kinase
  • Hypertonicity
  • Kidney
  • Mitogen-activated protein kinase
  • Signal transduction

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Urea and NaCl differentially regulate FAK and RAFTK/PYK2 in mIMCD3 renal medullary cells. / Zhang, Zheng; Avraham, Hava; Cohen, David.

In: American Journal of Physiology - Renal Physiology, Vol. 275, No. 3 44-3, 09.1998.

Research output: Contribution to journalArticle

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