Upregulation of brain-derived neurotrophic factor expression in nodose ganglia and the lower brainstem of hypertensive rats

Anke Vermehren-Schmaedick, Victoria K. Jenkins, Hui ya Hsieh, Alexandra L. Brown, Mollie P. Page, Virginia Brooks, Agnieszka Balkowiec

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Hypertension leads to structural and functional changes at baroreceptor synapses in the medial nucleus tractus solitarius (NTS), but the underlying molecular mechanisms remain unknown. Our previous studies show that brain-derived neurotrophic factor (BDNF) is abundantly expressed by rat nodose ganglion (NG) neurons, including baroreceptor afferents and their central terminals in the medial NTS. We hypothesized that hypertension leads to upregulation of BDNF expression in NG neurons. To test this hypothesis, we used two mechanistically distinct models of hypertension, the spontaneously hypertensive rat (SHR) and the deoxycorticosterone acetate (DOCA)-salt rat. Young adult SHRs, whose blood pressure was significantly elevated compared with age-matched Wistar-Kyoto (WKY) control rats, exhibited dramatic upregulation of BDNF mRNA and protein in the NG. BDNF transcripts from exon 4, known to be regulated by activity, and exon 9 (protein-coding region) showed the largest increases. Electrical stimulation of dispersed NG neurons with patterns that mimic baroreceptor activity during blood pressure elevations led to increases in BDNF mRNA that were also mediated through promoter 4. The increase in BDNF content of the NG in vivo was associated with a significant increase in the percentage of BDNF-immunoreactive NG neurons. Moreover, upregulation of BDNF in cell bodies of NG neurons was accompanied by a significant increase in BDNF in the NTS region, the primary central target of NG afferents. A dramatic increase in BDNF in the NG was also detected in DOCA-salt hypertensive rats. Together, our study identifies BDNF as a candidate molecular mediator of activity-dependent changes atbaroafferent synapses during hypertension.

Original languageEnglish (US)
Pages (from-to)220-229
Number of pages10
JournalJournal of Neuroscience Research
Volume91
Issue number2
DOIs
StatePublished - Feb 2013

Fingerprint

Nodose Ganglion
Brain-Derived Neurotrophic Factor
Brain Stem
Up-Regulation
Pressoreceptors
Solitary Nucleus
Neurons
Hypertension
Desoxycorticosterone
Synapses
Exons
Acetates
Salts
Blood Pressure
Messenger RNA
Inbred WKY Rats
Nerve Growth Factors
Inbred SHR Rats
Electric Stimulation
Open Reading Frames

Keywords

  • Baroreceptor
  • Blood pressure
  • DOCA
  • Nodose ganglion
  • SHR

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Upregulation of brain-derived neurotrophic factor expression in nodose ganglia and the lower brainstem of hypertensive rats. / Vermehren-Schmaedick, Anke; Jenkins, Victoria K.; Hsieh, Hui ya; Brown, Alexandra L.; Page, Mollie P.; Brooks, Virginia; Balkowiec, Agnieszka.

In: Journal of Neuroscience Research, Vol. 91, No. 2, 02.2013, p. 220-229.

Research output: Contribution to journalArticle

Vermehren-Schmaedick, Anke ; Jenkins, Victoria K. ; Hsieh, Hui ya ; Brown, Alexandra L. ; Page, Mollie P. ; Brooks, Virginia ; Balkowiec, Agnieszka. / Upregulation of brain-derived neurotrophic factor expression in nodose ganglia and the lower brainstem of hypertensive rats. In: Journal of Neuroscience Research. 2013 ; Vol. 91, No. 2. pp. 220-229.
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