Up-regulation of WNT-4 signaling and dosage-sensitive sex reversal in humans

Brian K. Jordan, Mansoor Mohammed, Saunders T. Ching, Emmanuèle Délot, Xiao Ning Chen, Phoebe Dewing, Amanda Swain, P. Nagesh Rao, B. Rafael Elejalde, Eric Vilain

Research output: Contribution to journalArticlepeer-review

259 Scopus citations

Abstract

Wnt-4, a member of the Wnt family of locally acting secreted growth factors, is the first signaling molecule shown to influence the sex-determination cascade. In mice, a targeted deletion of Wnt-4 causes the masculinization of XX pups. Therefore, WNT-4, the human homologue of murine Wnt-4, is a strong candidate gene for sex-reversal phenotypes in humans. In this article, we show that, in testicular Sertoli and Leydig cells, Wnt-4 up-regulates Dax1, a gene known to antagonize the testis-determining factor, Sry. Furthermore, we elucidate a possible mechanism for human XY sex reversal associated with a 1p31-p35 duplication including WNT-4. Overexpression of WNT-4 leads to up-regulation of DAX1, which results in an XY female phenotype. Thus, WNT-4, a novel sex-determining gene, and DAX1 play a concerted role in both the control of female development and the prevention of testes formation. These observations suggest that mammalian sex determination is sensitive to dosage, at multiple steps in its pathway.

Original languageEnglish (US)
Pages (from-to)1102-1109
Number of pages8
JournalAmerican Journal of Human Genetics
Volume68
Issue number5
DOIs
StatePublished - 2001
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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