Up-regulation of insulin-like growth factor-I (IGF-I) receptor gene expression in patients with reduced serum IGF-I levels

R. Eshet, H. Werner, B. Klinger, A. Silbergeld, Z. Laron, D. LeRoith, C. T. Roberts

    Research output: Contribution to journalArticle

    43 Scopus citations

    Abstract

    We have analysed the expression of the IGF-I receptor gene in lymphocytes of patients with low levels of circulating IGF-I (four patients with siolated GH deficiency (IGHD) and one Laron-type dwarf (LTD)) in comparison with a control group exhibiting normal serum IGF-I levels and endocrine profiles. 125I-Labelled IGF-I binding assays were performed on erythrocytes to determine the number of IGF-I binding sites per cell and their dissociation constants. Erythrocytes from patients with IGHD or LTD contained significantly (P = 0.002) more receptors per cell (10.9 ± 3.1 binding sites/cell), with a reduced affinity (K(d) = 0.49 ± 0.05 nM), than erythrocytes from controls (2.0 ± 0.4 sites/cell; K(d) = 0.14 nM). The levels of IGF-I receptor mRNA in circulating lymphocytes were determined by an RNA template-specific reverse transcription/polymerase chain reaction method. There was a statistically significant increase in IGF-I receptor mRNA levels in lymphocytes from patients with LTD or IGHD when compared with controls (3108.1 ± 775.9 vs 576.0 ± 465.7 arbitrary units, P = 0.006). The increased level of IGF-I binding due to increased IGF-I receptor gene expression may represent a compensatory up-regulation process activated in response to the low levels of IGF-I in the circulation of patients with LTD or IGHD.

    Original languageEnglish (US)
    Pages (from-to)115-120
    Number of pages6
    JournalJournal of molecular endocrinology
    Volume10
    Issue number2
    DOIs
    StatePublished - Jan 1 1993

    ASJC Scopus subject areas

    • Molecular Biology
    • Endocrinology

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