Untargeted Metabolomic Screen Reveals Changes in Human Plasma Metabolite Profiles Following Consumption of Fresh Broccoli Sprouts

Lauren Housley, Armando Alcazar Magana, Anna Hsu, Laura M. Beaver, Carmen P. Wong, Jan F. Stevens, Jaewoo Choi, Yuan Jiang, Deborah Bella, David E. Williams, Claudia S. Maier, Jackilen (Jackie) Shannon, Roderick H. Dashwood, Emily Ho

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Scope: Several lines of evidence suggest that the consumption of cruciferous vegetables is beneficial to human health. Yet, underlying mechanisms and key molecular targets that are involved with achieving these benefits in humans are still not fully understood. To accelerate this research, we conduct a human study to identify potential molecular targets of crucifers for further study. This study aims to characterize plasma metabolite profiles in humans before and after consuming fresh broccoli sprouts (a rich dietary source of bioactive sulforaphane). Methods and results: Ten healthy adults consume fresh broccoli sprouts (containing 200 μmol sulforaphane equivalents) at time 0 and provide blood samples at 0, 3, 6, 12, 24, and 48 h. An untargeted metabolomics screen reveals that levels of several plasma metabolites are significantly different before and after sprout intake, including fatty acids (14:0, 14:1, 16:0, 16:1, 18:0, and 18:1), glutathione, glutamine, cysteine, dehydroepiandrosterone, and deoxyuridine monophosphate. Evaluation of all time points is conducted using paired t-test (R software) and repeated measures analysis of variance for a within-subject design (Progenesis QI). Conclusion: This investigation identifies several potential molecular targets of crucifers that may aid in studying established and emerging health benefits of consuming cruciferous vegetables and related bioactive compounds.

Original languageEnglish (US)
JournalMolecular Nutrition and Food Research
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

sprouts (food)
Metabolomics
Brassica
metabolomics
broccoli
metabolites
Brassicaceae
Vegetables
prasterone
vegetable consumption
glutamine
Deoxyuridine
cysteine
Dehydroepiandrosterone
human health
glutathione
Insurance Benefits
analysis of variance
Glutamine
vegetables

Keywords

  • Broccoli sprouts
  • Human
  • Metabolomics
  • Plasma
  • Sulforaphane

ASJC Scopus subject areas

  • Biotechnology
  • Food Science

Cite this

Untargeted Metabolomic Screen Reveals Changes in Human Plasma Metabolite Profiles Following Consumption of Fresh Broccoli Sprouts. / Housley, Lauren; Magana, Armando Alcazar; Hsu, Anna; Beaver, Laura M.; Wong, Carmen P.; Stevens, Jan F.; Choi, Jaewoo; Jiang, Yuan; Bella, Deborah; Williams, David E.; Maier, Claudia S.; Shannon, Jackilen (Jackie); Dashwood, Roderick H.; Ho, Emily.

In: Molecular Nutrition and Food Research, 01.01.2018.

Research output: Contribution to journalArticle

Housley, L, Magana, AA, Hsu, A, Beaver, LM, Wong, CP, Stevens, JF, Choi, J, Jiang, Y, Bella, D, Williams, DE, Maier, CS, Shannon, JJ, Dashwood, RH & Ho, E 2018, 'Untargeted Metabolomic Screen Reveals Changes in Human Plasma Metabolite Profiles Following Consumption of Fresh Broccoli Sprouts', Molecular Nutrition and Food Research. https://doi.org/10.1002/mnfr.201700665
Housley, Lauren ; Magana, Armando Alcazar ; Hsu, Anna ; Beaver, Laura M. ; Wong, Carmen P. ; Stevens, Jan F. ; Choi, Jaewoo ; Jiang, Yuan ; Bella, Deborah ; Williams, David E. ; Maier, Claudia S. ; Shannon, Jackilen (Jackie) ; Dashwood, Roderick H. ; Ho, Emily. / Untargeted Metabolomic Screen Reveals Changes in Human Plasma Metabolite Profiles Following Consumption of Fresh Broccoli Sprouts. In: Molecular Nutrition and Food Research. 2018.
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