Unidirectional transport across cultured ovine amniotic epithelial cell monolayer

Cecilia Y. Cheung, Robert A. Brace

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Objective: To determine whether ovine amniotic cells in monolayer culture exhibit unidirectional transport. Methods: Permeability of 14C-inulin was measured in two directions across ovine amniotic cells grown to confluence on cell culture inserts. Results: Permeability was 0.69 + 0.17 (SE) μl/min/cm2 from the top of the insert where the cells were attached to the bottom and 0.11 + 0.02 μl/min/cm2 in the reverse direction (P < 0.001). Permeability was similar in the two directions for chorionic cells and umbilical vein endothelial cells. Addition of vascular endothelial growth factor did not alter permeability. Conclusions: Transcellular transport in ovine amniotic monolayers is directionally specific. The transport ratio of 6.2:1 for 14C-inulin is comparable to the in vivo ratio of 4.7:1 for the ovine intramembranous pathway and is consistent with passive diffusion in both directions and vesicular transport in one direction across the monolayer at 5.2 times the rate of passive diffusion.

Original languageEnglish (US)
Pages (from-to)1054-1058
Number of pages5
JournalReproductive Sciences
Volume15
Issue number10
DOIs
StatePublished - Dec 2008

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Keywords

  • Amniotic fluid volume
  • Amniotic membrane
  • Amniotic permeability
  • Intramembranous pathway
  • Vesicular transport

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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