Two replication initiation sites on R-plasmid DNA

Jorge H. Crosa, Linda K. Luttropp, Fred Heffron, Stanley Falkow

    Research output: Contribution to journalArticle

    31 Scopus citations

    Abstract

    Replicating DNA molecules of a deletion mutant of the conjugative R-plasmid R 6 K are cleaved at a single site by the EcoRI restriction endonuclease. Electron microscope examination and measurements of the EcoRI treated replicative intermediate molecules indicate that replication can be initiated at two sites on the plasmid DNA molecule. The two sites are located at about 23 and 39% of total length, respectively, from the EcoRI cleavage site. About 5% of the replicating molecules use both replication initiation sites simultaneously.

    Original languageEnglish (US)
    Pages (from-to)39-50
    Number of pages12
    JournalMGG Molecular & General Genetics
    Volume140
    Issue number1
    DOIs
    StatePublished - Mar 1 1975

    ASJC Scopus subject areas

    • Genetics

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  • Cite this

    Crosa, J. H., Luttropp, L. K., Heffron, F., & Falkow, S. (1975). Two replication initiation sites on R-plasmid DNA. MGG Molecular & General Genetics, 140(1), 39-50. https://doi.org/10.1007/BF00268987