Two replication initiation sites on R-plasmid DNA

Jorge H. Crosa, Linda K. Luttropp, Fred Heffron, Stanley Falkow

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Replicating DNA molecules of a deletion mutant of the conjugative R-plasmid R 6 K are cleaved at a single site by the EcoRI restriction endonuclease. Electron microscope examination and measurements of the EcoRI treated replicative intermediate molecules indicate that replication can be initiated at two sites on the plasmid DNA molecule. The two sites are located at about 23 and 39% of total length, respectively, from the EcoRI cleavage site. About 5% of the replicating molecules use both replication initiation sites simultaneously.

Original languageEnglish (US)
Pages (from-to)39-50
Number of pages12
JournalMGG Molecular & General Genetics
Volume140
Issue number1
DOIs
StatePublished - Mar 1975
Externally publishedYes

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R Factors
Deoxyribonuclease EcoRI
DNA
DNA Restriction Enzymes
Plasmids
Electrons

ASJC Scopus subject areas

  • Genetics

Cite this

Crosa, J. H., Luttropp, L. K., Heffron, F., & Falkow, S. (1975). Two replication initiation sites on R-plasmid DNA. MGG Molecular & General Genetics, 140(1), 39-50. https://doi.org/10.1007/BF00268987

Two replication initiation sites on R-plasmid DNA. / Crosa, Jorge H.; Luttropp, Linda K.; Heffron, Fred; Falkow, Stanley.

In: MGG Molecular & General Genetics, Vol. 140, No. 1, 03.1975, p. 39-50.

Research output: Contribution to journalArticle

Crosa, JH, Luttropp, LK, Heffron, F & Falkow, S 1975, 'Two replication initiation sites on R-plasmid DNA', MGG Molecular & General Genetics, vol. 140, no. 1, pp. 39-50. https://doi.org/10.1007/BF00268987
Crosa, Jorge H. ; Luttropp, Linda K. ; Heffron, Fred ; Falkow, Stanley. / Two replication initiation sites on R-plasmid DNA. In: MGG Molecular & General Genetics. 1975 ; Vol. 140, No. 1. pp. 39-50.
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