Two new members of the OmpR superfamily detected by homology to a sensor-binding core domain.

T. L. Timme, C. B. Lawrence, Robb Moses

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The OmpR superfamily includes proteins that act as transcriptional regulators of operons that respond to environmental stimuli. A homologous domain near the N-terminus, termed a sensor-binding core domain, is thought to play a role in recognition of a signal transduction protein. We have identified two previously unrecognized members of this regulator family of proteins: a 23.8-kd protein transcribed from the uvrC transcription unit and the PgtA gene product, which is a phosphoglycerate transport regulatory protein. The sensor-binding core domain is also present in four proteins that regulate bacterial sporulation and chemotaxis. The 23.8-kd protein also has sequence similarity to elongation factor Tu and two regulatory proteins: HtpR, the heat-shock regulatory protein, and TraJ, a regulator of expression of genes involved in conjugation. There is a 77-amino acid region near the C-terminus of the 23.8-kd protein that has 30% similarity with a 28.1-kd protein coded for by an open reading frame 5' to the reading frame of the 23.8-kd protein in the uvrC transcription unit. Genetic distance analysis of amino acid sequences of proteins with a sensor-binding core domain suggests that the 23.8-kd protein and the chemotaxis regulatory proteins are distantly related to the other regulatory proteins in the OmpR superfamily.

Original languageEnglish (US)
Pages (from-to)545-552
Number of pages8
JournalJournal of Molecular Evolution
Volume28
Issue number6
StatePublished - Jun 1989
Externally publishedYes

Fingerprint

homology
sensor
protein
regulatory proteins
Sensors
Proteins
proteins
chemotaxis
Chemotaxis
transcription (genetics)
Transcription
phosphoglyceric acids
bacterial proteins
transport proteins
Peptide Elongation Factor Tu
operon
sporulation
heat shock proteins
Reading Frames
amino acid

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)
  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Genetics
  • Molecular Biology
  • Genetics(clinical)

Cite this

Two new members of the OmpR superfamily detected by homology to a sensor-binding core domain. / Timme, T. L.; Lawrence, C. B.; Moses, Robb.

In: Journal of Molecular Evolution, Vol. 28, No. 6, 06.1989, p. 545-552.

Research output: Contribution to journalArticle

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