Two insulin-like growth factor I messenger RNAs are expressed in human liver

P. Rotwein

Research output: Contribution to journalArticle

215 Citations (Scopus)

Abstract

Through use of a synthetic oligonucleotide probe, human insulin-like growth factor I (IGF-I) cDNA clones were isolated from a liver library. Two types of cDNAs were defined by restriction enzyme analysis and DNA sequencing. Both encode IGF-I precursors of either 195 or 153 amino acids. The two predicted protein precursors are identical from their amino terminus to a lysine residue 16 codons beyond the IGF-I sequence, and then they diverge. Both cDNAs predict additional unique carboxyl-terminal extension peptides. Since there is only one IGF-I gene in the human genome, the finding of two different cDNAs suggests that alternative RNA processing plays a role in IGF-I gene expression. The functions of the different extension peptides remain to be elucidated.

Original languageEnglish (US)
Pages (from-to)77-81
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number1
StatePublished - 1986
Externally publishedYes

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Insulin-Like Growth Factor I
Messenger RNA
Complementary DNA
Liver
Restriction Mapping
Peptides
Protein Precursors
Oligonucleotide Probes
Human Genome
DNA Sequence Analysis
Codon
Lysine
Clone Cells
immune RNA
RNA
Gene Expression
Amino Acids
Genes

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Two insulin-like growth factor I messenger RNAs are expressed in human liver. / Rotwein, P.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, No. 1, 1986, p. 77-81.

Research output: Contribution to journalArticle

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