Two insulin-like growth factor I messenger RNAs are expressed in human liver

P. Rotwein

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Abstract

Through use of a synthetic oligonucleotide probe, human insulin-like growth factor I (IGF-I) cDNA clones were isolated from a liver library. Two types of cDNAs were defined by restriction enzyme analysis and DNA sequencing. Both encode IGF-I precursors of either 195 or 153 amino acids. The two predicted protein precursors are identical from their amino terminus to a lysine residue 16 codons beyond the IGF-I sequence, and then they diverge. Both cDNAs predict additional unique carboxyl-terminal extension peptides. Since there is only one IGF-I gene in the human genome, the finding of two different cDNAs suggests that alternative RNA processing plays a role in IGF-I gene expression. The functions of the different extension peptides remain to be elucidated.

Original languageEnglish (US)
Pages (from-to)77-81
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number1
DOIs
StatePublished - Jan 1 1986

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