Tumors alter inflammation and impair dermal wound healing in female mice

Leah M. Pyter, Yasmin Husain, Humberto Calero, Daniel B. McKim, Hsin Yun Lin, Jonathan P. Godbout, John F. Sheridan, Christopher G. Engeland, Phillip T. Marucha

Research output: Research - peer-reviewArticle

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Abstract

Tissue repair is an integral component of cancer treatment (e.g., due to surgery, chemotherapy, radiation). Previous work has emphasized the immunosuppressive effects of tumors on adaptive immunity and has shown that surgery incites cancer metastases. However, the extent to which and how tumors may alter the clinically-relevant innate immune process of wound healing remains an untapped potential area of improvement for treatment, quality of life, and ultimately, mortality of cancer patients. In this study, 3.5 mm full-Thickness dermal excisional wounds were placed on the dorsum of immunocompetent female mice with and without non-malignant flank AT-84 murine oral squamous cell carcinomas. Wound closure rate, inflammatory cell number and inflammatory signaling in wounds, and circulating myeloid cell concentrations were compared between tumor-bearing and tumor-free mice. Tumors delayed wound closure, suppressed inflammatory signaling, and altered myeloid cell trafficking in wounds. An in vitro scratch "wounding" assay of adult dermal fibroblasts treated with tumor cell-conditioned media supported the in vivo findings. This study demonstrates that tumors are sufficient to disrupt fundamental and clinically-relevant innate immune functions. The understanding of these underlying mechanisms provides potential for therapeutic interventions capable of improving the treatment of cancer while reducing morbidities and mortality.

LanguageEnglish (US)
Article numbere0161537
JournalPLoS One
Volume11
Issue number8
DOIs
StatePublished - Aug 1 2016

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Wound Healing
Inflammation
Skin
Neoplasms
tissue repair
inflammation
neoplasms
mice
Tumors
animal injuries
Wounds and Injuries
Therapeutics
cells
Surgery
surgery
Myeloid Cells
Mortality
Bearings (structural)
Oncology
Chemotherapy

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Pyter, L. M., Husain, Y., Calero, H., McKim, D. B., Lin, H. Y., Godbout, J. P., ... Marucha, P. T. (2016). Tumors alter inflammation and impair dermal wound healing in female mice. PLoS One, 11(8), [e0161537]. DOI: 10.1371/journal.pone.0161537

Tumors alter inflammation and impair dermal wound healing in female mice. / Pyter, Leah M.; Husain, Yasmin; Calero, Humberto; McKim, Daniel B.; Lin, Hsin Yun; Godbout, Jonathan P.; Sheridan, John F.; Engeland, Christopher G.; Marucha, Phillip T.

In: PLoS One, Vol. 11, No. 8, e0161537, 01.08.2016.

Research output: Research - peer-reviewArticle

Pyter, LM, Husain, Y, Calero, H, McKim, DB, Lin, HY, Godbout, JP, Sheridan, JF, Engeland, CG & Marucha, PT 2016, 'Tumors alter inflammation and impair dermal wound healing in female mice' PLoS One, vol 11, no. 8, e0161537. DOI: 10.1371/journal.pone.0161537
Pyter LM, Husain Y, Calero H, McKim DB, Lin HY, Godbout JP et al. Tumors alter inflammation and impair dermal wound healing in female mice. PLoS One. 2016 Aug 1;11(8). e0161537. Available from, DOI: 10.1371/journal.pone.0161537
Pyter, Leah M. ; Husain, Yasmin ; Calero, Humberto ; McKim, Daniel B. ; Lin, Hsin Yun ; Godbout, Jonathan P. ; Sheridan, John F. ; Engeland, Christopher G. ; Marucha, Phillip T./ Tumors alter inflammation and impair dermal wound healing in female mice. In: PLoS One. 2016 ; Vol. 11, No. 8.
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