Tubulin and microtubules in cochlear hair cells: Comparative immunocytochemistry and ultrastructure

Peter Steyger, D. N. Furness, C. M. Hackney, G. P. Richardson

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

The distribution of tubulin has been investigated in surface preparations of the guinea pig organ of Corti using indirect immunofluorescence microscopy. Two different monoclonal antibodies to tubulin produce similar distinct patterns of labelling in hair cells. Labelling is greater in inner hair cells than outer hair cells. It occurs in rings around the cell apex, and in a meshwork below and channels through, the cuticular plate. In outer hair cells from the apical region of the cochlea, labelling occurs around the location of a basalward protrusion of the cuticular plate. These patterns correlate with the location of microtubules observed using transmission electron microscopy. A large patch of labelling occurs on the strial side of the cell corresponding to the largest channel through the cuticular plate and the kinociliary basal body. Strands of labelling are seen running parallel to the long axis of the cell between the subcuticular and synaptic region. Many more of these strands are seen in the inner hair cell than the outer hair cell and may correspond to tracks of microtubules transporting neurotransmitter vesicles or other organelles. In outer hair cells, intense labelling and many microtubules are seen in the subnuclear region. The possible roles of the different microtubule arrangements are discussed.

Original languageEnglish (US)
Pages (from-to)1-16
Number of pages16
JournalHearing Research
Volume42
Issue number1
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Outer Auditory Hair Cells
Auditory Hair Cells
Tubulin
Inner Auditory Hair Cells
Microtubules
Immunohistochemistry
Basal Bodies
Organ of Corti
Cochlea
Indirect Fluorescent Antibody Technique
Transmission Electron Microscopy
Fluorescence Microscopy
Organelles
Neurotransmitter Agents
Guinea Pigs
Monoclonal Antibodies

Keywords

  • Cochlea
  • Hair cell
  • Immunofluorescence
  • Microtubules
  • Tubulin

ASJC Scopus subject areas

  • Sensory Systems

Cite this

Tubulin and microtubules in cochlear hair cells : Comparative immunocytochemistry and ultrastructure. / Steyger, Peter; Furness, D. N.; Hackney, C. M.; Richardson, G. P.

In: Hearing Research, Vol. 42, No. 1, 1989, p. 1-16.

Research output: Contribution to journalArticle

Steyger, Peter ; Furness, D. N. ; Hackney, C. M. ; Richardson, G. P. / Tubulin and microtubules in cochlear hair cells : Comparative immunocytochemistry and ultrastructure. In: Hearing Research. 1989 ; Vol. 42, No. 1. pp. 1-16.
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