Tryptophan Cu(I)-π interaction fine-tunes the metal binding properties of the bacterial metallochaperone CusF

Isabell R. Loftin, Ninian Blackburn, Megan M. McEvoy

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The periplasmic metallochaperone CusF coordinates Cu(I) and Ag(I) through a unique site consisting of a Met2His motif as well as a Cu(I)-π interaction between a nearby tryptophan, W44, and the metal ion. Through mutational analyses we investigate here the role that W44 in CusF plays in metal coordination. Nuclear magnetic resonance spectra show that the specificity of CusF for Cu(I) and Ag(I) is not altered by mutation of W44. X-ray absorption spectroscopy studies reveal that W44 protects the bound Cu(I) from oxidation as well as from adventitious ligands. Competition assays demonstrate that W44 does not significantly contribute to the affinity of CusF for metal, but that substitution of W44 by methionine, which forms a fourth Cu(I) ligand, substantially increases the affinity. These studies indicate that W44 is important in maintaining a moderate-affinity and solvent-shielded three-coordinate environment for Cu(I), which has implications for the function of CusF as a metallochaperone.

Original languageEnglish (US)
Pages (from-to)905-912
Number of pages8
JournalJournal of Biological Inorganic Chemistry
Volume14
Issue number6
DOIs
StatePublished - 2009

Fingerprint

Metallochaperones
Tryptophan
Metals
Ligands
X ray absorption spectroscopy
X-Ray Absorption Spectroscopy
Methionine
Metal ions
Assays
Substitution reactions
Nuclear magnetic resonance
Oxidation
Magnetic Resonance Spectroscopy
Ions
Mutation

Keywords

  • Copper
  • Copper coordination
  • Metallochaperone

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

Cite this

Tryptophan Cu(I)-π interaction fine-tunes the metal binding properties of the bacterial metallochaperone CusF. / Loftin, Isabell R.; Blackburn, Ninian; McEvoy, Megan M.

In: Journal of Biological Inorganic Chemistry, Vol. 14, No. 6, 2009, p. 905-912.

Research output: Contribution to journalArticle

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AB - The periplasmic metallochaperone CusF coordinates Cu(I) and Ag(I) through a unique site consisting of a Met2His motif as well as a Cu(I)-π interaction between a nearby tryptophan, W44, and the metal ion. Through mutational analyses we investigate here the role that W44 in CusF plays in metal coordination. Nuclear magnetic resonance spectra show that the specificity of CusF for Cu(I) and Ag(I) is not altered by mutation of W44. X-ray absorption spectroscopy studies reveal that W44 protects the bound Cu(I) from oxidation as well as from adventitious ligands. Competition assays demonstrate that W44 does not significantly contribute to the affinity of CusF for metal, but that substitution of W44 by methionine, which forms a fourth Cu(I) ligand, substantially increases the affinity. These studies indicate that W44 is important in maintaining a moderate-affinity and solvent-shielded three-coordinate environment for Cu(I), which has implications for the function of CusF as a metallochaperone.

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