Translational control in sea urchin eggs and embryos

Initiation is rate limiting in blastula stage embryos

M. B. Hille, D. C. Hall, Z. Yablonka-Reuveni, Michael Danilchik, R. T. Moon

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

To determine whether initiation is rate-limiting in protein synthesis during the embryogenesis of sea urchins, polyribosome profiles of unfertilized eggs and cleavage, blastula and prism stage embryos were examined after incubation of the eggs and embryos in the presence and absence of low amounts of emetine, an inhibitor of polypeptide elongation. The ribosomes were radioactively labeled with [3H]uridine by injection of the adults during oogenesis so that we could monitor emetine-dependent shifts of monoribosomes to polyribosomes. Although initiation is not rate limiting in unfertilized eggs or 2- to 16-cell embryos of Strongylocentrotus purpuratus, it is rate limiting in blastula and prism embryos. We suggest that initiation becomes rate limiting to allow the selective translation of certain classes of mRNA during later development.

Original languageEnglish (US)
Pages (from-to)241-249
Number of pages9
JournalDevelopmental Biology
Volume86
Issue number1
DOIs
StatePublished - 1981
Externally publishedYes

Fingerprint

Blastula
Sea Urchins
Eggs
Embryonic Structures
Emetine
Polyribosomes
Ovum
Strongylocentrotus purpuratus
Oogenesis
Uridine
Ribosomes
Embryonic Development
Messenger RNA
Peptides
Injections
Proteins

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Translational control in sea urchin eggs and embryos : Initiation is rate limiting in blastula stage embryos. / Hille, M. B.; Hall, D. C.; Yablonka-Reuveni, Z.; Danilchik, Michael; Moon, R. T.

In: Developmental Biology, Vol. 86, No. 1, 1981, p. 241-249.

Research output: Contribution to journalArticle

Hille, M. B. ; Hall, D. C. ; Yablonka-Reuveni, Z. ; Danilchik, Michael ; Moon, R. T. / Translational control in sea urchin eggs and embryos : Initiation is rate limiting in blastula stage embryos. In: Developmental Biology. 1981 ; Vol. 86, No. 1. pp. 241-249.
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