TY - JOUR
T1 - Translational control in sea urchin eggs and embryos
T2 - Initiation is rate limiting in blastula stage embryos
AU - Hille, M. B.
AU - Hall, D. C.
AU - Yablonka-Reuveni, Z.
AU - Danilchik, M. V.
AU - Moon, R. T.
N1 - Funding Information:
We thank Dr. Charles D. Laird for the use of the Numonics Electronic Graphics Calculator and scintillation counter. This research was supported by NIH Grant H 11070, University of Washington Graduate School Research Project Fund, and Institutional Cancer Grant IN-26 from the American Cancer Society. MVD and RTM were supported by NIH National Research Service Awards GM 07270 and IT 32 HD 07183, resepctively.
PY - 1981/8
Y1 - 1981/8
N2 - To determine whether initiation is rate-limiting in protein synthesis during the embryogenesis of sea urchins, polyribosome profiles of unfertilized eggs and cleavage, blastula and prism stage embryos were examined after incubation of the eggs and embryos in the presence and absence of low amounts of emetine, an inhibitor of polypeptide elongation. The ribosomes were radioactively labeled with [3H]uridine by injection of the adults during oogenesis so that we could monitor emetine-dependent shifts of monoribosomes to polyribosomes. Although initiation is not rate limiting in unfertilized eggs or 2- to 16-cell embryos of Strongylocentrotus purpuratus, it is rate limiting in blastula and prism embryos. We suggest that initiation becomes rate limiting to allow the selective translation of certain classes of mRNA during later development.
AB - To determine whether initiation is rate-limiting in protein synthesis during the embryogenesis of sea urchins, polyribosome profiles of unfertilized eggs and cleavage, blastula and prism stage embryos were examined after incubation of the eggs and embryos in the presence and absence of low amounts of emetine, an inhibitor of polypeptide elongation. The ribosomes were radioactively labeled with [3H]uridine by injection of the adults during oogenesis so that we could monitor emetine-dependent shifts of monoribosomes to polyribosomes. Although initiation is not rate limiting in unfertilized eggs or 2- to 16-cell embryos of Strongylocentrotus purpuratus, it is rate limiting in blastula and prism embryos. We suggest that initiation becomes rate limiting to allow the selective translation of certain classes of mRNA during later development.
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U2 - 10.1016/0012-1606(81)90336-5
DO - 10.1016/0012-1606(81)90336-5
M3 - Article
C2 - 6793424
AN - SCOPUS:0019797078
SN - 0012-1606
VL - 86
SP - 241
EP - 249
JO - Developmental Biology
JF - Developmental Biology
IS - 1
ER -