Transient transfection of oligodendrocyte progenitors by electroporation

W. H.H. Krueger, D. L. Madison, S. E. Pfeiffer

Research output: Contribution to journalReview articlepeer-review

6 Scopus citations

Abstract

The transient transfection of transgenes into oligodendrocytes offers an important tool for studying the function of proteins during myelin formation. Currently established procedures, however, have generally resulted in low survival rates and low levels of uptake of the transgene into primary oligodendrocyte progenitors. We describe an electropotation method which yields transient transfection of oligodendrocyte progenitors of up to 10-15% of the surviving cells, and provides approximately 104 surviving, transfected cells per electroporation reaction. In recent applications transgene expression persisted as the transfected progenitors progressed through subsequent stages of the oligodendrocyte lineage. This technique is expected to facilitate the study of the function of key proteins and lipids during the development of primary cultured oligodendrocytes.

Original languageEnglish (US)
Pages (from-to)421-426
Number of pages6
JournalNeurochemical Research
Volume23
Issue number3
DOIs
StatePublished - Mar 24 1998

Keywords

  • Electroporation
  • Gene transfer
  • Oligodendrocytes
  • Transfection

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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