Transgenic zebrafish for studying nervous system development and regeneration

D. Goldman, Mark Hankin, Z. Li, X. Dai, J. Ding

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

α 1 tubulin gene expression is induced in the developing and regenerating CNS of vertebrates. Therefore, α 1 tubulin gene expression may serve as a good probe for mechanisms underlying CNS development and regeneration. One approach to identify these mechanisms is to work backwards from the genome. This requires identification of cd tubulin DNA-sequences that mediate its developmental and regeneration-dependent expression pattern. Therefore, we generated transgenic zebrafish harboring a fragment of the α 1 tubulin gene driving green fluorescent protein expression (GFP). In these fish, and similar to the endogenous gene, transgene expression was dramatically induced in the developing and regenerating nervous system. Although transgene expression generally declined during maturation of the nervous system, robust GFP expression was maintained in progenitor cells in the retinal periphery, lining brain ventricles and surrounding the central canal of the spinal cord. When these cells were cultured in vitro they divided and gave rise to new neurons. We also show that optic nerve crush in adult fish re-induced transgene expression in retinal ganglion cells. These studies identified a relatively small region of the α 1 tubulin promoter that mediates its regulated expression pattern in developing and adult fish. This promoter will be extremely useful to investigators interested in targeting gene expression to the developing or regenerating nervous system. As adult transgenic fish maintain transgene expression in neural progenitors, these fish also provide a valuable resource of labeled adult neural progenitor cells that can be studied in vivo or in vitro. Finally, these fish should provide a unique in vivo system for investigating mechanisms mediating CNS development and regeneration.

Original languageEnglish (US)
Pages (from-to)21-33
Number of pages13
JournalTransgenic Research
Volume10
Issue number1
DOIs
StatePublished - Mar 12 2001
Externally publishedYes

Fingerprint

neurodevelopment
Zebrafish
Danio rerio
Nervous System
Regeneration
tubulin
Fishes
genetically modified organisms
Tubulin
Transgenes
transgenes
fish
nervous system
Gene Expression
Green Fluorescent Proteins
green fluorescent protein
gene expression
stem cells
Stem Cells
Tubulin Modulators

Keywords

  • α1 tubulin
  • Gene expression
  • Nervous system development
  • Neural stem cells
  • Optic nerve regeneration
  • Zebrafish

ASJC Scopus subject areas

  • Biotechnology
  • Animal Science and Zoology
  • Genetics
  • Agronomy and Crop Science

Cite this

Transgenic zebrafish for studying nervous system development and regeneration. / Goldman, D.; Hankin, Mark; Li, Z.; Dai, X.; Ding, J.

In: Transgenic Research, Vol. 10, No. 1, 12.03.2001, p. 21-33.

Research output: Contribution to journalArticle

Goldman, D. ; Hankin, Mark ; Li, Z. ; Dai, X. ; Ding, J. / Transgenic zebrafish for studying nervous system development and regeneration. In: Transgenic Research. 2001 ; Vol. 10, No. 1. pp. 21-33.
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