Transgenic introduction of androgen receptor into estrogen-receptor-, progesterone-receptor-, and androgen-receptor-negative breast cancer cells renders them responsive to hormonal manipulation

Jennifer R. Garreau, Patrick Muller, Rodney Pommier, Su Ellen Pommier

    Research output: Contribution to journalArticle

    18 Scopus citations

    Abstract

    Background: Estrogen-receptor (ER)-, progesterone-receptor (PR)-, and androgen-receptor (AR)-negative breast cancer cells are unaffected by treatment with dehydroepiandrosterone-sulfate (DHEAS) and an aromatase inhibitor (AI). We hypothesized that cell growth would be inhibited with DHEAS/AI treatment after successful transfection of an AR expression vector. Methods: ER/PR/AR-negative breast cancer cells were transfected with an AR expression vector and treated with DHEAS/AI for 2 days. Growth inhibition of these cells was compared with that of transfected cells treated with only AI or with nontransfected cells treated with DHEAS/AI. Mann-Whitney U test was used to determine statistical significance. Results: Cell death rates of 53.5% (P = .001) and 40.1% (P = .006) were seen in transfected cells treated with DHEAS/AI compared with controls for days 1 and 2, respectively. Nontransfected cells were unaffected by treatment. Comments: ER/PR/AR-negative cells transfected with AR were killed by DHEAS/AI treatment, providing evidence that AR is responsible for this effect. This provides the first AR-targeted hormonal therapy for ER breast cancer.

    Original languageEnglish (US)
    Pages (from-to)576-580
    Number of pages5
    JournalAmerican journal of surgery
    Volume191
    Issue number5
    DOIs
    StatePublished - May 1 2006

    Keywords

    • Androgen receptor
    • Aromatase inhibitor
    • Breast cancer
    • Dehydroepiandrosterone-sulfate

    ASJC Scopus subject areas

    • Surgery

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