Transepithelial transport of guinea pig gastric mucous cell monolayers.

Michael Rutten, D. Rattner, W. Silen

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Abstract

Guinea pig gastric mucous epithelial cells were isolated, enriched, cultured in collagen cups, and put into Ussing chambers for electrophysiological studies. The cultured mucous cell monolayers, which retain the morphology of surface cells found in the intact tissue, had a maximal resistance (R) of 272 +/- 12 omega X cm2 and a potential difference (PD) of -3.8 +/- 0.4 mV (apical negative) between 4 and 10 days later (n = 33). The current-voltage and conductance-concentration relationships of the cultures were both nonlinear (n = 12). In addition, NaCl concentration gradients across the monolayer also gave asymmetrical and nonlinear dilution potentials, with the side of lower chemical potential always becoming electrically negative (n = 10). Calculation of the average Cl(-)-to-Na+ permeability ratio at pH 7.4 was 1.35, indicating a slightly greater conductance of anions over cations. Amiloride (0.1-1.0 microM) had no effect on PD or R when given from the apical or basal side (n = 18), but at higher concentrations (0.1-1.0 mM) there was a decrease in the PD. 4,4'-Diisothiocyanostilbene-2,2'-disulfonic acid at 10(-4) M increased R from the apical side only (n = 14), and acetazolamide at 5 X 10(-4) M reduced the PD to -0.5 mV (n = 8). Only ouabain at 10(-4) M from the serosal side was effective in reducing the monolayer PD to zero. This culture preparation will prove useful for future studies in determining specific functions for this gastric cell type and how those functions relate to barrier function in the stomach.

Original languageEnglish (US)
JournalThe American journal of physiology
Volume249
Issue number5 Pt 1
StatePublished - Nov 1985
Externally publishedYes

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Stomach
Guinea Pigs
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
Acetazolamide
Amiloride
Ouabain
Anions
Cations
Cultured Cells
Permeability
Collagen
Epithelial Cells

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Transepithelial transport of guinea pig gastric mucous cell monolayers. / Rutten, Michael; Rattner, D.; Silen, W.

In: The American journal of physiology, Vol. 249, No. 5 Pt 1, 11.1985.

Research output: Contribution to journalArticle

Rutten, Michael ; Rattner, D. ; Silen, W. / Transepithelial transport of guinea pig gastric mucous cell monolayers. In: The American journal of physiology. 1985 ; Vol. 249, No. 5 Pt 1.
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