Transcriptomic and epigenomic characterization of the developing bat wing

Walter L. Eckalbar, Stephen A. Schlebusch, Mandy K. Mason, Zoe Gill, Ash V. Parker, Betty M. Booker, Sierra Nishizaki, Christiane Muswamba-Nday, Elizabeth Terhune, Kimberly A. Nevonen, Nadja Makki, Tara Friedrich, Julia E. VanderMeer, Katherine S. Pollard, Lucia Carbone, Jeff D. Wall, Nicola Illing, Nadav Ahituv

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Bats are the only mammals capable of powered flight, but little is known about the genetic determinants that shape their wings. Here we generated a genome for Miniopterus natalensis and performed RNA-seq and ChIP-seq (H3K27ac and H3K27me3) analyses on its developing forelimb and hindlimb autopods at sequential embryonic stages to decipher the molecular events that underlie bat wing development. Over 7,000 genes and several long noncoding RNAs, including Tbx5-as1 and Hottip, were differentially expressed between forelimb and hindlimb, and across different stages. ChIP-seq analysis identified thousands of regions that are differentially modified in forelimb and hindlimb. Comparative genomics found 2,796 bat-accelerated regions within H3K27ac peaks, several of which cluster near limb-associated genes. Pathway analyses highlighted multiple ribosomal proteins and known limb patterning signaling pathways as differentially regulated and implicated increased forelimb mesenchymal condensation in differential growth. In combination, our work outlines multiple genetic components that likely contribute to bat wing formation, providing insights into this morphological innovation.

Original languageEnglish (US)
Pages (from-to)528-536
Number of pages9
JournalNature Genetics
Volume48
Issue number5
DOIs
StatePublished - May 1 2016

Fingerprint

Forelimb
Epigenomics
Hindlimb
Extremities
Long Noncoding RNA
Ribosomal Proteins
Genomics
Genes
Mammals
Genome
RNA
Growth

ASJC Scopus subject areas

  • Genetics

Cite this

Eckalbar, W. L., Schlebusch, S. A., Mason, M. K., Gill, Z., Parker, A. V., Booker, B. M., ... Ahituv, N. (2016). Transcriptomic and epigenomic characterization of the developing bat wing. Nature Genetics, 48(5), 528-536. https://doi.org/10.1038/ng.3537

Transcriptomic and epigenomic characterization of the developing bat wing. / Eckalbar, Walter L.; Schlebusch, Stephen A.; Mason, Mandy K.; Gill, Zoe; Parker, Ash V.; Booker, Betty M.; Nishizaki, Sierra; Muswamba-Nday, Christiane; Terhune, Elizabeth; Nevonen, Kimberly A.; Makki, Nadja; Friedrich, Tara; VanderMeer, Julia E.; Pollard, Katherine S.; Carbone, Lucia; Wall, Jeff D.; Illing, Nicola; Ahituv, Nadav.

In: Nature Genetics, Vol. 48, No. 5, 01.05.2016, p. 528-536.

Research output: Contribution to journalArticle

Eckalbar, WL, Schlebusch, SA, Mason, MK, Gill, Z, Parker, AV, Booker, BM, Nishizaki, S, Muswamba-Nday, C, Terhune, E, Nevonen, KA, Makki, N, Friedrich, T, VanderMeer, JE, Pollard, KS, Carbone, L, Wall, JD, Illing, N & Ahituv, N 2016, 'Transcriptomic and epigenomic characterization of the developing bat wing', Nature Genetics, vol. 48, no. 5, pp. 528-536. https://doi.org/10.1038/ng.3537
Eckalbar WL, Schlebusch SA, Mason MK, Gill Z, Parker AV, Booker BM et al. Transcriptomic and epigenomic characterization of the developing bat wing. Nature Genetics. 2016 May 1;48(5):528-536. https://doi.org/10.1038/ng.3537
Eckalbar, Walter L. ; Schlebusch, Stephen A. ; Mason, Mandy K. ; Gill, Zoe ; Parker, Ash V. ; Booker, Betty M. ; Nishizaki, Sierra ; Muswamba-Nday, Christiane ; Terhune, Elizabeth ; Nevonen, Kimberly A. ; Makki, Nadja ; Friedrich, Tara ; VanderMeer, Julia E. ; Pollard, Katherine S. ; Carbone, Lucia ; Wall, Jeff D. ; Illing, Nicola ; Ahituv, Nadav. / Transcriptomic and epigenomic characterization of the developing bat wing. In: Nature Genetics. 2016 ; Vol. 48, No. 5. pp. 528-536.
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