Titration of a vaccine stock preparation of human immunodeficiency virus type 1(SF2) in cultured lymphocytes and in chimpanzees

Krishna K. Murthy, E. Kathy Cobb, Zahwa El-Amad, Henry Ortega, Frank C. Hsueh, William Satterfield, D. Rick Lee, Marcia L. Kalish, Nancy L. Haigwood, Ronald C. Kennedy, Kathelyn S. Steimer, Alan Schultz, Jay A. Levy

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    Abstract

    A large stock preparation of the HIV-1(SF2) isolate has been derived after serial passage in human peripheral blood mononuclear cells (PBMCs). This viral stock has a titer of 104.9 TCID50 in human PBMCs and 104.2 TCID50 in chimpanzee PBMCs. By inoculation into animals the 50% chimpanzee infections dose titer was found to be about 102.3. Virus isolation from animals was achieved on most occasions within 1-4 weeks after inoculation and then became transient. Viral RNA and DNA PCR analyses confirmed the virus infection of the chimpanzees. Anti-HIV antibody levels in the inoculated animals ranged from 1:400 to 1:6400 as measured by ELISA. About 680 vials of this stock preparation, frozen at -190°C, are available for future studies of vaccines and antiviral therapies.

    Original languageEnglish (US)
    Pages (from-to)1341-1348
    Number of pages8
    JournalAIDS Research and Human Retroviruses
    Volume12
    Issue number14
    DOIs
    StatePublished - Sep 20 1996

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    ASJC Scopus subject areas

    • Immunology
    • Virology
    • Infectious Diseases

    Cite this

    Murthy, K. K., Cobb, E. K., El-Amad, Z., Ortega, H., Hsueh, F. C., Satterfield, W., Lee, D. R., Kalish, M. L., Haigwood, N. L., Kennedy, R. C., Steimer, K. S., Schultz, A., & Levy, J. A. (1996). Titration of a vaccine stock preparation of human immunodeficiency virus type 1(SF2) in cultured lymphocytes and in chimpanzees. AIDS Research and Human Retroviruses, 12(14), 1341-1348. https://doi.org/10.1089/aid.1996.12.1341