A large stock preparation of the HIV-1(SF2) isolate has been derived after serial passage in human peripheral blood mononuclear cells (PBMCs). This viral stock has a titer of 104.9 TCID50 in human PBMCs and 104.2 TCID50 in chimpanzee PBMCs. By inoculation into animals the 50% chimpanzee infections dose titer was found to be about 102.3. Virus isolation from animals was achieved on most occasions within 1-4 weeks after inoculation and then became transient. Viral RNA and DNA PCR analyses confirmed the virus infection of the chimpanzees. Anti-HIV antibody levels in the inoculated animals ranged from 1:400 to 1:6400 as measured by ELISA. About 680 vials of this stock preparation, frozen at -190°C, are available for future studies of vaccines and antiviral therapies.
ASJC Scopus subject areas
- Infectious Diseases