Tissue-specific expression of the rat galanin gene.

L. M. Kaplan, Eliot Spindel, K. J. Isselbacher, W. W. Chin

Research output: Contribution to journalArticle

228 Citations (Scopus)

Abstract

We have isolated and characterized cDNAs encoding rat galanin from a cDNA library prepared from rat hypothalamic tissue. Analysis of these clones reveals that rat galanin is synthesized initially as part of a 124-amino acid precursor that includes a signal peptide, galanin (29 amino acids), and a 60-amino acid galanin mRNA-associated peptide. In the precursor, galanin includes a C-terminal glycine and is flanked on each side by dibasic tryptic cleavage sites. The deduced amino acid sequence of rat galanin is 90% similar to porcine galanin, with all three amino acid differences in the C-terminal heptapeptide. The predicted galanin mRNA-associated peptide includes a 35-amino acid sequence that is 78% similar to the previously reported porcine analogue. This sequence is set off by a single basic tryptic cleavage site and includes a 17-amino acid region that is nearly identical to the porcine counterpart. The high interspecies conservation suggests a biological role for this putative peptide. Blot hybridization analysis using rat genomic DNA is consistent with a single galanin-encoding gene. RNA blot analysis of total RNA prepared from rat tissues reveals a single band of hybridizing mRNA that is approximately 900 nucleotides long. Rat galanin mRNA is located predominantly in the central nervous system and gastrointestinal tract. Highest central nervous system concentrations are found in the hypothalamus, with lower levels in the cortex and brainstem. Gastrointestinal rat galanin mRNA is most abundant in the duodenum, with progressively lower concentrations in the stomach, small intestine, and colon.

Original languageEnglish (US)
Pages (from-to)1065-1069
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number4
StatePublished - Feb 1988
Externally publishedYes

Fingerprint

Galanin
Genes
Amino Acids
Messenger RNA
Swine
Peptides
Amino Acid Sequence
Central Nervous System
RNA
Protein Sorting Signals
Gene Library
Duodenum
Glycine
Hypothalamus
Brain Stem
Small Intestine
Gastrointestinal Tract
Stomach
Colon
Nucleotides

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Tissue-specific expression of the rat galanin gene. / Kaplan, L. M.; Spindel, Eliot; Isselbacher, K. J.; Chin, W. W.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, No. 4, 02.1988, p. 1065-1069.

Research output: Contribution to journalArticle

@article{cb83af3d4b8241b8a74936bf71e2c45c,
title = "Tissue-specific expression of the rat galanin gene.",
abstract = "We have isolated and characterized cDNAs encoding rat galanin from a cDNA library prepared from rat hypothalamic tissue. Analysis of these clones reveals that rat galanin is synthesized initially as part of a 124-amino acid precursor that includes a signal peptide, galanin (29 amino acids), and a 60-amino acid galanin mRNA-associated peptide. In the precursor, galanin includes a C-terminal glycine and is flanked on each side by dibasic tryptic cleavage sites. The deduced amino acid sequence of rat galanin is 90{\%} similar to porcine galanin, with all three amino acid differences in the C-terminal heptapeptide. The predicted galanin mRNA-associated peptide includes a 35-amino acid sequence that is 78{\%} similar to the previously reported porcine analogue. This sequence is set off by a single basic tryptic cleavage site and includes a 17-amino acid region that is nearly identical to the porcine counterpart. The high interspecies conservation suggests a biological role for this putative peptide. Blot hybridization analysis using rat genomic DNA is consistent with a single galanin-encoding gene. RNA blot analysis of total RNA prepared from rat tissues reveals a single band of hybridizing mRNA that is approximately 900 nucleotides long. Rat galanin mRNA is located predominantly in the central nervous system and gastrointestinal tract. Highest central nervous system concentrations are found in the hypothalamus, with lower levels in the cortex and brainstem. Gastrointestinal rat galanin mRNA is most abundant in the duodenum, with progressively lower concentrations in the stomach, small intestine, and colon.",
author = "Kaplan, {L. M.} and Eliot Spindel and Isselbacher, {K. J.} and Chin, {W. W.}",
year = "1988",
month = "2",
language = "English (US)",
volume = "85",
pages = "1065--1069",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "4",

}

TY - JOUR

T1 - Tissue-specific expression of the rat galanin gene.

AU - Kaplan, L. M.

AU - Spindel, Eliot

AU - Isselbacher, K. J.

AU - Chin, W. W.

PY - 1988/2

Y1 - 1988/2

N2 - We have isolated and characterized cDNAs encoding rat galanin from a cDNA library prepared from rat hypothalamic tissue. Analysis of these clones reveals that rat galanin is synthesized initially as part of a 124-amino acid precursor that includes a signal peptide, galanin (29 amino acids), and a 60-amino acid galanin mRNA-associated peptide. In the precursor, galanin includes a C-terminal glycine and is flanked on each side by dibasic tryptic cleavage sites. The deduced amino acid sequence of rat galanin is 90% similar to porcine galanin, with all three amino acid differences in the C-terminal heptapeptide. The predicted galanin mRNA-associated peptide includes a 35-amino acid sequence that is 78% similar to the previously reported porcine analogue. This sequence is set off by a single basic tryptic cleavage site and includes a 17-amino acid region that is nearly identical to the porcine counterpart. The high interspecies conservation suggests a biological role for this putative peptide. Blot hybridization analysis using rat genomic DNA is consistent with a single galanin-encoding gene. RNA blot analysis of total RNA prepared from rat tissues reveals a single band of hybridizing mRNA that is approximately 900 nucleotides long. Rat galanin mRNA is located predominantly in the central nervous system and gastrointestinal tract. Highest central nervous system concentrations are found in the hypothalamus, with lower levels in the cortex and brainstem. Gastrointestinal rat galanin mRNA is most abundant in the duodenum, with progressively lower concentrations in the stomach, small intestine, and colon.

AB - We have isolated and characterized cDNAs encoding rat galanin from a cDNA library prepared from rat hypothalamic tissue. Analysis of these clones reveals that rat galanin is synthesized initially as part of a 124-amino acid precursor that includes a signal peptide, galanin (29 amino acids), and a 60-amino acid galanin mRNA-associated peptide. In the precursor, galanin includes a C-terminal glycine and is flanked on each side by dibasic tryptic cleavage sites. The deduced amino acid sequence of rat galanin is 90% similar to porcine galanin, with all three amino acid differences in the C-terminal heptapeptide. The predicted galanin mRNA-associated peptide includes a 35-amino acid sequence that is 78% similar to the previously reported porcine analogue. This sequence is set off by a single basic tryptic cleavage site and includes a 17-amino acid region that is nearly identical to the porcine counterpart. The high interspecies conservation suggests a biological role for this putative peptide. Blot hybridization analysis using rat genomic DNA is consistent with a single galanin-encoding gene. RNA blot analysis of total RNA prepared from rat tissues reveals a single band of hybridizing mRNA that is approximately 900 nucleotides long. Rat galanin mRNA is located predominantly in the central nervous system and gastrointestinal tract. Highest central nervous system concentrations are found in the hypothalamus, with lower levels in the cortex and brainstem. Gastrointestinal rat galanin mRNA is most abundant in the duodenum, with progressively lower concentrations in the stomach, small intestine, and colon.

UR - http://www.scopus.com/inward/record.url?scp=0023955973&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023955973&partnerID=8YFLogxK

M3 - Article

VL - 85

SP - 1065

EP - 1069

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 4

ER -