Thyrotropin-releasing hormone stimulates phosphorylation of the epidermal growth factor receptor in GH3 pituitary cells

Ying Hong Wang, Shall F. Jue, Richard Maurer

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

TRH has been found to stimulate tyrosine phosphorylation of the epidermal growth factor (EGF) receptor. A specific EGF receptor kinase inhibitor, tyrphostin AG1478, substantially reduced TRH-stimulated tyrosine phosphorylation of the EGF receptor. TRH-induced EGF receptor phosphorylation was found to lead to the recruitment of the adapter proteins Grb2 and Shc. TRH treatment also led to phosphorylation of the related receptor tyrosine kinase, HER2. HER2 activation likely contributes to downstream signaling events and enhances EGF receptor action. TRH-induced tyrosine phosphorylation of the EGF receptor was reduced by incubation with a protein kinase C (PKC) kinase inhibitor, GF109203X. EGF receptor phosphorylation was required for full TRH-induced activation of mitogen-activated protein kinase (MAPK) and stimulation of specific transcriptional responses.

Original languageEnglish (US)
Pages (from-to)1328-1337
Number of pages10
JournalMolecular Endocrinology
Volume14
Issue number9
StatePublished - 2000

Fingerprint

Thyrotropin-Releasing Hormone
Epidermal Growth Factor Receptor
Phosphorylation
Tyrosine
Receptor Protein-Tyrosine Kinases
Mitogen-Activated Protein Kinases

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Thyrotropin-releasing hormone stimulates phosphorylation of the epidermal growth factor receptor in GH3 pituitary cells. / Wang, Ying Hong; Jue, Shall F.; Maurer, Richard.

In: Molecular Endocrinology, Vol. 14, No. 9, 2000, p. 1328-1337.

Research output: Contribution to journalArticle

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