The vermicelli and capellini handling tests: Simple quantitative measures of dexterous forepaw function in rats and mice

Kelly A. Tennant, Aaron L. Asay, Rachel P. Allred, Angela Ozburn, Jeffrey A. Kleim, Theresa A. Jones

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Previous characterizations of rodent eating behavior have revealed that they use coordinated forepaw movements to manipulate food pieces. We have extended upon this work to develop a simple quantitative measure of forepaw dexterity that is sensitive to lateralized impairments and age-dependent changes. Rodents learn skillful forepaw and digit movements to manage thin pasta pieces, which they eagerly consume. We have previously described methods for quantifying vermicelli handling in rats and showed that the measures are very sensitive to forelimb impairments resulting from unilateral ischemic lesions, middle cerebral artery occlusions and unilateral striatal dopamine depletion [Allred, R.P., Adkins, D.L., Woodlee, M.T., Husbands, L.C., Maldonado M.A., Kane, J.R., Schallert, T. & Jones, T.A. The Vermicelli Handling Test: a simple quantitative measure of dexterous forepaw function in rats. J. Neurosci. Methods 170, 229-244 (2008)]. Here we present a more detailed protocol for this test in rats and compare it with a newly developed version for mice, the Capellini Handling Test. Rats and mice are videotaped while handling short lengths of uncooked vermicelli or capellini pasta, respectively, with a camera positioned to optimize the view of paw movements. Slow motion video playback allows for the identification of forepaw adjustments, defined as any distinct removal and replacement of the paw, or of any number of digits, on the pasta piece after eating commences. Forepaw adjustments per piece are averaged over trials per each testing session. Repeated testing permits sensitive quantitative analysis of changes in forepaw dexterity over time. Protocols for pre-testing habituation and handling practice, as well as procedures for characterizing atypical handling patterns, are described. Because rats and mice perform the pasta handling tests slightly differently, species-specific differences in administration and scoring of these tests are highlighted. All animal use was in accordance with protocols approved by the University of Texas at Austin Animal Care and Use Committee.

Original languageEnglish (US)
Article numbere2076
JournalJournal of Visualized Experiments
Issue number41
DOIs
StatePublished - Jan 1 2010
Externally publishedYes

Fingerprint

Rats
Social Adjustment
Rodentia
Animals
Testing
Animal Care Committees
Corpus Striatum
Forelimb
Middle Cerebral Artery Infarction
Feeding Behavior
Spouses
Dopamine
Eating
Cameras
Food
Chemical analysis

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

The vermicelli and capellini handling tests : Simple quantitative measures of dexterous forepaw function in rats and mice. / Tennant, Kelly A.; Asay, Aaron L.; Allred, Rachel P.; Ozburn, Angela; Kleim, Jeffrey A.; Jones, Theresa A.

In: Journal of Visualized Experiments, No. 41, e2076, 01.01.2010.

Research output: Contribution to journalArticle

Tennant, Kelly A. ; Asay, Aaron L. ; Allred, Rachel P. ; Ozburn, Angela ; Kleim, Jeffrey A. ; Jones, Theresa A. / The vermicelli and capellini handling tests : Simple quantitative measures of dexterous forepaw function in rats and mice. In: Journal of Visualized Experiments. 2010 ; No. 41.
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