The uterine lumen of the pregnant guinea-pig contains a large macrophage population

William Sype, Karen Lentfer, Dwight J. Kimberly, Mari Kay Smith, Lori Van Meter, Kent Thornburg

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    Cellular constituents of the uterine lumen were investigated. Fourteen pregnant sows of 40+ days' gestation were anaesthetized and naturally occurring peritoneal fluid was collected. A uterine horn was delivered and 0.25 ml Gey's solution injected into the uterine lumen to collect free cells. The fluid was aspirated into a syringe and the cells extracted, counted and prepared for phagocytosis experiments and microscopy. The cells were stained with alpha-naphthyl-acetate-esterase (ANAE) to determine the fraction that was non-specific esterase-positive, a feature of mononuclear phagocytes. Differential cells counts were also made. Both uterine and peritoneal compartments yielded large numbers of cells (greater than 106/ml). Peritoneal fluid cells were 47 ± 6 per cent (SD) macrophages and 49 ± 6 per cent eosinophils (the remainder being 'other" cells); 47 ± 6 per cent also stained positively for ANAE. Uterine cells were 78 ± 12 per cent macrophages, the remainder being mostly lymphocytes (18 ± II per cent); 85 ± 13 per cent stained positively with ANAE. Electron microscopy of the uterine cells confirmed that most had morphology consistent with being mononuclear phagocytes. Uterine and peritoneal cells phagocytized carbon particles and yeast cells when incubated at 37°C. The origin and role of this macrophage population is unknown but uterine lumenal macrophages may be present to remove antigen-antibody complexes thus facilitating uptake of maternally derived IgG by the fetal yolk sac.

    Original languageEnglish (US)
    Pages (from-to)125-135
    Number of pages11
    JournalPlacenta
    Volume10
    Issue number2
    DOIs
    StatePublished - 1989

    Fingerprint

    Guinea Pigs
    Macrophages
    Naphthol AS D Esterase
    Population
    Ascitic Fluid
    Phagocytes
    Cell Count
    Carboxylesterase
    Yolk Sac
    Syringes
    Antigen-Antibody Complex
    Phagocytosis
    Eosinophils
    Microscopy
    Electron Microscopy
    Carbon
    Immunoglobulin G
    Yeasts
    Lymphocytes
    Pregnancy

    ASJC Scopus subject areas

    • Obstetrics and Gynecology

    Cite this

    The uterine lumen of the pregnant guinea-pig contains a large macrophage population. / Sype, William; Lentfer, Karen; Kimberly, Dwight J.; Smith, Mari Kay; Van Meter, Lori; Thornburg, Kent.

    In: Placenta, Vol. 10, No. 2, 1989, p. 125-135.

    Research output: Contribution to journalArticle

    Sype, W, Lentfer, K, Kimberly, DJ, Smith, MK, Van Meter, L & Thornburg, K 1989, 'The uterine lumen of the pregnant guinea-pig contains a large macrophage population', Placenta, vol. 10, no. 2, pp. 125-135. https://doi.org/10.1016/0143-4004(89)90035-0
    Sype, William ; Lentfer, Karen ; Kimberly, Dwight J. ; Smith, Mari Kay ; Van Meter, Lori ; Thornburg, Kent. / The uterine lumen of the pregnant guinea-pig contains a large macrophage population. In: Placenta. 1989 ; Vol. 10, No. 2. pp. 125-135.
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    abstract = "Cellular constituents of the uterine lumen were investigated. Fourteen pregnant sows of 40+ days' gestation were anaesthetized and naturally occurring peritoneal fluid was collected. A uterine horn was delivered and 0.25 ml Gey's solution injected into the uterine lumen to collect free cells. The fluid was aspirated into a syringe and the cells extracted, counted and prepared for phagocytosis experiments and microscopy. The cells were stained with alpha-naphthyl-acetate-esterase (ANAE) to determine the fraction that was non-specific esterase-positive, a feature of mononuclear phagocytes. Differential cells counts were also made. Both uterine and peritoneal compartments yielded large numbers of cells (greater than 106/ml). Peritoneal fluid cells were 47 ± 6 per cent (SD) macrophages and 49 ± 6 per cent eosinophils (the remainder being 'other{"} cells); 47 ± 6 per cent also stained positively for ANAE. Uterine cells were 78 ± 12 per cent macrophages, the remainder being mostly lymphocytes (18 ± II per cent); 85 ± 13 per cent stained positively with ANAE. Electron microscopy of the uterine cells confirmed that most had morphology consistent with being mononuclear phagocytes. Uterine and peritoneal cells phagocytized carbon particles and yeast cells when incubated at 37°C. The origin and role of this macrophage population is unknown but uterine lumenal macrophages may be present to remove antigen-antibody complexes thus facilitating uptake of maternally derived IgG by the fetal yolk sac.",
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