The Unique Cysteine of F-ATP Synthase OSCP Subunit Participates in Modulation of the Permeability Transition Pore

Michela Carraro, Kristen Jones, Geppo Sartori, Marco Schiavone, Salvatore Antonucci, Roza Kucharczyk, Jean Paul di Rago, Cinzia Franchin, Giorgio Arrigoni, Michael Forte, Paolo Bernardi

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The mitochondrial permeability transition pore (PTP) is a Ca2+-activated channel that plays a key role in cell death. Thiol oxidation facilitates PTP opening, yet the targets and molecular mechanisms still await a definition. Here, we investigate the role of C141 of F-ATP synthase oligomycin sensitivity conferral protein (OSCP) subunit in PTP modulation by oxidation. We find that the OSCP C141S mutation confers resistance to PTP opening and cell death by diamide and MitoParaquat only when cyclophilin D (CyPD) has been ablated, a protective role that can be explained by CyPD shielding C141 from oxidants. The mutation decreases apoptosis in zebrafish embryos, indicating that this OSCP residue is involved in development. Site-directed mutagenesis in yeast suggests that other conserved cysteines in the α, γ, and c subunits of F-ATP synthase are not involved in PTP modulation. Thus, OSCP provides a strategic site that regulates PTP opening by the interplay between CyPD (un)binding and thiol oxidation-reduction.

Original languageEnglish (US)
Article number108095
JournalCell Reports
Volume32
Issue number9
DOIs
StatePublished - Sep 1 2020

Keywords

  • F-ATP synthase
  • OSCP
  • cyclophilin D
  • cysteine
  • mitochondria
  • oxidation
  • permeability transition pore

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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