The transferrin receptor cytoplasmic domain determines its rate of transport through the biosynthetic pathway and its susceptibility to cleavage early in the pathway

Elizabeth A. Rutledge, Isabelle Gaston, Barbara J. Root, Timothy E. McGraw, Caroline Enns

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The soluble human transferrin receptor (TfR) found in blood is the result of a proteolytic cleavage occurring in the ectodomain of the receptor close to the transmembrane domain at Arg-100. We have discovered another cleavage site between Gly-91 and Val-92 even closer to the transmembrane domain. Cleavage at Gly-91 differs markedly from the normal cleavage site. It occurs when the entire cytoplasmic portion or the proximal 31 amino acids of the transmembrane domain are deleted. A soluble disulfide-bonded dimer of the TfR is released into the medium in contrast to the cleavage at Arg-100 where a dimer lacking intersubunit disulfide bonds is released. Whereas the cleavage at Arg-100 is generated by cycling through the endosomal system, pulse-chase experiments indicate that cleavage at Gly-91 occurs predominantly during the biosynthesis of the receptor. Pulse-chase analysis of the biosynthesis of mutant TfRs that lack the membrane-proximal cytoplasmic domain show that they exit the endoglycosidase H-sensitive compartment at a slower rate than the wild type TfR. These results suggest that the cytoplasmic domain influences the trafficking of the TfR either by influencing the folding of the ectodomain or by providing a positive signal for its transport through the biosynthetic pathway.

Original languageEnglish (US)
Pages (from-to)12169-12175
Number of pages7
JournalJournal of Biological Chemistry
Volume273
Issue number20
DOIs
StatePublished - May 15 1998

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Transferrin Receptors
Biosynthetic Pathways
Biosynthesis
Disulfides
Dimers
Glycoside Hydrolases
Contrast Media
Blood
Cell Membrane
Membranes
Amino Acids
Experiments

ASJC Scopus subject areas

  • Biochemistry

Cite this

The transferrin receptor cytoplasmic domain determines its rate of transport through the biosynthetic pathway and its susceptibility to cleavage early in the pathway. / Rutledge, Elizabeth A.; Gaston, Isabelle; Root, Barbara J.; McGraw, Timothy E.; Enns, Caroline.

In: Journal of Biological Chemistry, Vol. 273, No. 20, 15.05.1998, p. 12169-12175.

Research output: Contribution to journalArticle

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