The time course of glutamate in the synaptic cleft

John D. Clements, Robin A J Lester, Gang Tong, Craig Jahr, Gary Westbrook

Research output: Contribution to journalArticle

716 Citations (Scopus)

Abstract

The peak concentration and rate of clearance of neurotransmitter from the synaptic cleft are important determinants of synaptic function, yet the neurotransmitter concentration time course is unknown atl synapses in the brain. The time course of free glutamate in the cleft was estimated by kinetic analysis of the displacement of a rapidly dissociating competitive antagonist from N-methyl-D-aspartate (NMDA) receptors during synaptic transmission. Glutamate peaked at 1.1 millimolar and decayed with a time constant of 1.2 milliseconds at cultured hippocampal synapses. This time course implies that transmitter saturates postsynaptic NMDA receptors. However, glutamate dissociates much more rapidly from α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors. Thus, the time course of free glutamate predicts that dissociation contributes to the decay of the AMPA receptor-mediated postsynaptic current.

Original languageEnglish (US)
Pages (from-to)1498-1501
Number of pages4
JournalScience
Volume258
Issue number5087
StatePublished - Nov 27 1992

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Glutamic Acid
AMPA Receptors
N-Methyl-D-Aspartate Receptors
Synapses
Neurotransmitter Agents
Synaptic Potentials
Synaptic Transmission
Acids
Brain

ASJC Scopus subject areas

  • General

Cite this

Clements, J. D., Lester, R. A. J., Tong, G., Jahr, C., & Westbrook, G. (1992). The time course of glutamate in the synaptic cleft. Science, 258(5087), 1498-1501.

The time course of glutamate in the synaptic cleft. / Clements, John D.; Lester, Robin A J; Tong, Gang; Jahr, Craig; Westbrook, Gary.

In: Science, Vol. 258, No. 5087, 27.11.1992, p. 1498-1501.

Research output: Contribution to journalArticle

Clements, JD, Lester, RAJ, Tong, G, Jahr, C & Westbrook, G 1992, 'The time course of glutamate in the synaptic cleft', Science, vol. 258, no. 5087, pp. 1498-1501.
Clements JD, Lester RAJ, Tong G, Jahr C, Westbrook G. The time course of glutamate in the synaptic cleft. Science. 1992 Nov 27;258(5087):1498-1501.
Clements, John D. ; Lester, Robin A J ; Tong, Gang ; Jahr, Craig ; Westbrook, Gary. / The time course of glutamate in the synaptic cleft. In: Science. 1992 ; Vol. 258, No. 5087. pp. 1498-1501.
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