The steady state concentration gradients of an electron dense marker (Ferritin) in the 3 layered hemochorial placenta of the rabbit

Kent Thornburg, J. J. Faber

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    10 Citations (Scopus)

    Abstract

    Ferritin was injected into the fetal or the maternal circulation of 27 to 29 day pregnant rabbits. After the occurrence of a quasi steady state, the placentas were prepared for electron microscopy. Ferritin particles were counted in the electron micrographs in the fetal capillaries, in the maternal blood spaces, and in the 2 interstitial compartments of the 3 layered placenta. Under the circumstances of the experiments (excessively elevated plasma ferritin concentrations), no evidence was found for nondiffusional transport of radiolabeled ferritin. Comparison of the standing concentration gradients in the placentas, recorded after maternal and after fetal injection, showed that the interstitial spaces 'excluded' ferritin; the plasma interstitial space ferritin partition coefficients were 10 in the basement membrane space and 3 in the space between the cyto and syncytiotrophoblasts. 55% Of the total concentration gradient across the rabbit placenta occurred across the fetal endothelium, about 45% across the cytotrophoblast, and less than 5% across the syncytiotrophoblast. These figures are believed to reflect the relative contributions of these 3 layers to the total diffusional resistance in the rabbit placenta. When compared to previous data on the relative contributions of these 3 layers for small ions and molecules, the present data lead to the conclusion that discrimination of molecular size is a function of the fetal capillary endothelium alone.

    Original languageEnglish (US)
    Pages (from-to)912-925
    Number of pages14
    JournalJournal of Clinical Investigation
    Volume58
    Issue number4
    StatePublished - 1976

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    Ferritins
    Placenta
    Electrons
    Rabbits
    Trophoblasts
    Mothers
    Vascular Endothelium
    Basement Membrane
    Endothelium
    Electron Microscopy
    Ions
    Injections

    ASJC Scopus subject areas

    • Medicine(all)

    Cite this

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    title = "The steady state concentration gradients of an electron dense marker (Ferritin) in the 3 layered hemochorial placenta of the rabbit",
    abstract = "Ferritin was injected into the fetal or the maternal circulation of 27 to 29 day pregnant rabbits. After the occurrence of a quasi steady state, the placentas were prepared for electron microscopy. Ferritin particles were counted in the electron micrographs in the fetal capillaries, in the maternal blood spaces, and in the 2 interstitial compartments of the 3 layered placenta. Under the circumstances of the experiments (excessively elevated plasma ferritin concentrations), no evidence was found for nondiffusional transport of radiolabeled ferritin. Comparison of the standing concentration gradients in the placentas, recorded after maternal and after fetal injection, showed that the interstitial spaces 'excluded' ferritin; the plasma interstitial space ferritin partition coefficients were 10 in the basement membrane space and 3 in the space between the cyto and syncytiotrophoblasts. 55{\%} Of the total concentration gradient across the rabbit placenta occurred across the fetal endothelium, about 45{\%} across the cytotrophoblast, and less than 5{\%} across the syncytiotrophoblast. These figures are believed to reflect the relative contributions of these 3 layers to the total diffusional resistance in the rabbit placenta. When compared to previous data on the relative contributions of these 3 layers for small ions and molecules, the present data lead to the conclusion that discrimination of molecular size is a function of the fetal capillary endothelium alone.",
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    T1 - The steady state concentration gradients of an electron dense marker (Ferritin) in the 3 layered hemochorial placenta of the rabbit

    AU - Thornburg, Kent

    AU - Faber, J. J.

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    N2 - Ferritin was injected into the fetal or the maternal circulation of 27 to 29 day pregnant rabbits. After the occurrence of a quasi steady state, the placentas were prepared for electron microscopy. Ferritin particles were counted in the electron micrographs in the fetal capillaries, in the maternal blood spaces, and in the 2 interstitial compartments of the 3 layered placenta. Under the circumstances of the experiments (excessively elevated plasma ferritin concentrations), no evidence was found for nondiffusional transport of radiolabeled ferritin. Comparison of the standing concentration gradients in the placentas, recorded after maternal and after fetal injection, showed that the interstitial spaces 'excluded' ferritin; the plasma interstitial space ferritin partition coefficients were 10 in the basement membrane space and 3 in the space between the cyto and syncytiotrophoblasts. 55% Of the total concentration gradient across the rabbit placenta occurred across the fetal endothelium, about 45% across the cytotrophoblast, and less than 5% across the syncytiotrophoblast. These figures are believed to reflect the relative contributions of these 3 layers to the total diffusional resistance in the rabbit placenta. When compared to previous data on the relative contributions of these 3 layers for small ions and molecules, the present data lead to the conclusion that discrimination of molecular size is a function of the fetal capillary endothelium alone.

    AB - Ferritin was injected into the fetal or the maternal circulation of 27 to 29 day pregnant rabbits. After the occurrence of a quasi steady state, the placentas were prepared for electron microscopy. Ferritin particles were counted in the electron micrographs in the fetal capillaries, in the maternal blood spaces, and in the 2 interstitial compartments of the 3 layered placenta. Under the circumstances of the experiments (excessively elevated plasma ferritin concentrations), no evidence was found for nondiffusional transport of radiolabeled ferritin. Comparison of the standing concentration gradients in the placentas, recorded after maternal and after fetal injection, showed that the interstitial spaces 'excluded' ferritin; the plasma interstitial space ferritin partition coefficients were 10 in the basement membrane space and 3 in the space between the cyto and syncytiotrophoblasts. 55% Of the total concentration gradient across the rabbit placenta occurred across the fetal endothelium, about 45% across the cytotrophoblast, and less than 5% across the syncytiotrophoblast. These figures are believed to reflect the relative contributions of these 3 layers to the total diffusional resistance in the rabbit placenta. When compared to previous data on the relative contributions of these 3 layers for small ions and molecules, the present data lead to the conclusion that discrimination of molecular size is a function of the fetal capillary endothelium alone.

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