TY - JOUR
T1 - The Src substrate Tks5, podosomes (Invadopodia), and cancer cell invasion
AU - Courtneidge, S. A.
AU - Azucena, E. F.
AU - Pass, I.
AU - Seals, D. F.
AU - Tesfay, L.
PY - 2005
Y1 - 2005
N2 - Some years ago, we employed a screen of phage cDNA expression libraries to identify novel substrates of the protein tyrosine kinase Src. One of these, Tks5 (previously known as Fish), is a large scaffolding protein with an amino-terminal PX domain and five SH3 domains. In normal fibroblasts, Tks5 is cytoplasmic, but the protein is found in podosomes when the cells are transformed with Src. Using short interfering RNA technology, we have shown that Tks5 is required for podosome formation. Furthermore, cells with reduced Tks5 expression are poorly invasive through Matrigel. Tks5 is expressed and localized to podosomes in invasive human cancer cell lines and in tumor tissue, particularly breast cancers and melanomas. In these cells too, Tks5 is required for invasion. Our future work will focus on the identification of the binding partners of Tks5 that are responsible for podosome formation and invasion, and on determining the role of Tks5 in animal models of metastasis.
AB - Some years ago, we employed a screen of phage cDNA expression libraries to identify novel substrates of the protein tyrosine kinase Src. One of these, Tks5 (previously known as Fish), is a large scaffolding protein with an amino-terminal PX domain and five SH3 domains. In normal fibroblasts, Tks5 is cytoplasmic, but the protein is found in podosomes when the cells are transformed with Src. Using short interfering RNA technology, we have shown that Tks5 is required for podosome formation. Furthermore, cells with reduced Tks5 expression are poorly invasive through Matrigel. Tks5 is expressed and localized to podosomes in invasive human cancer cell lines and in tumor tissue, particularly breast cancers and melanomas. In these cells too, Tks5 is required for invasion. Our future work will focus on the identification of the binding partners of Tks5 that are responsible for podosome formation and invasion, and on determining the role of Tks5 in animal models of metastasis.
UR - http://www.scopus.com/inward/record.url?scp=33746469431&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33746469431&partnerID=8YFLogxK
U2 - 10.1101/sqb.2005.70.014
DO - 10.1101/sqb.2005.70.014
M3 - Article
C2 - 16869750
AN - SCOPUS:33746469431
SN - 0091-7451
VL - 70
SP - 167
EP - 171
JO - Cold Spring Harbor symposia on quantitative biology
JF - Cold Spring Harbor symposia on quantitative biology
ER -