The spread of Ras activity triggered by activation of a single dendritic spine

Christopher D. Harvey, Ryohei Yasuda, Haining Zhong, Karel Svoboda

Research output: Contribution to journalArticle

258 Citations (Scopus)

Abstract

In neurons, individual dendritic spines isolate N-methyl-D-aspartate (NMDA) receptor-mediated calcium ion (Ca2+) accumulations from the dendrite and other spines. However, the extent to which spines compartmentalize signaling events downstream of Ca2+ influx is not known. We combined two-photon fluorescence lifetime imaging with two-photon glutamate uncaging to image the activity of the small guanosine triphosphatase Ras after NMDA receptor activation at individual spines. Induction of long-term potentiation (LTP) triggered robust Ca2+-dependent Ras activation in single spines that decayed in ∼5 minutes. Ras activity spread over ∼10 micrometers of dendrite and invaded neighboring spines by diffusion. The spread of Ras-dependent signaling was necessary for the local regulation of the threshold for LTP induction. Thus, Ca2+-dependent synaptic signals can spread to couple multiple synapses on short stretches of dendrite.

Original languageEnglish (US)
Pages (from-to)136-140
Number of pages5
JournalScience
Volume321
Issue number5885
DOIs
StatePublished - Jul 4 2008
Externally publishedYes

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Dendritic Spines
Spine
Dendrites
Long-Term Potentiation
N-Methyl-D-Aspartate Receptors
Photons
Guanosine
Optical Imaging
Synapses
Glutamic Acid
Ions
Calcium
Neurons

ASJC Scopus subject areas

  • General

Cite this

The spread of Ras activity triggered by activation of a single dendritic spine. / Harvey, Christopher D.; Yasuda, Ryohei; Zhong, Haining; Svoboda, Karel.

In: Science, Vol. 321, No. 5885, 04.07.2008, p. 136-140.

Research output: Contribution to journalArticle

Harvey, Christopher D. ; Yasuda, Ryohei ; Zhong, Haining ; Svoboda, Karel. / The spread of Ras activity triggered by activation of a single dendritic spine. In: Science. 2008 ; Vol. 321, No. 5885. pp. 136-140.
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