The spread of Ras activity triggered by activation of a single dendritic spine

Christopher D. Harvey; Ryohei Yasuda; Haining Zhong; Karel Svoboda

doi:10.1126/science.1159675

The spread of Ras activity triggered by activation of a single dendritic spine

Christopher D. Harvey, Ryohei Yasuda, Haining Zhong, Karel Svoboda

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338 Scopus citations

Abstract

In neurons, individual dendritic spines isolate N-methyl-D-aspartate (NMDA) receptor-mediated calcium ion (Ca²⁺) accumulations from the dendrite and other spines. However, the extent to which spines compartmentalize signaling events downstream of Ca²⁺ influx is not known. We combined two-photon fluorescence lifetime imaging with two-photon glutamate uncaging to image the activity of the small guanosine triphosphatase Ras after NMDA receptor activation at individual spines. Induction of long-term potentiation (LTP) triggered robust Ca²⁺-dependent Ras activation in single spines that decayed in ∼5 minutes. Ras activity spread over ∼10 micrometers of dendrite and invaded neighboring spines by diffusion. The spread of Ras-dependent signaling was necessary for the local regulation of the threshold for LTP induction. Thus, Ca²⁺-dependent synaptic signals can spread to couple multiple synapses on short stretches of dendrite.

Original language	English (US)
Pages (from-to)	136-140
Number of pages	5
Journal	Science
Volume	321
Issue number	5885
DOIs	https://doi.org/10.1126/science.1159675
State	Published - Jul 4 2008
Externally published	Yes

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title = "The spread of Ras activity triggered by activation of a single dendritic spine",

abstract = "In neurons, individual dendritic spines isolate N-methyl-D-aspartate (NMDA) receptor-mediated calcium ion (Ca2+) accumulations from the dendrite and other spines. However, the extent to which spines compartmentalize signaling events downstream of Ca2+ influx is not known. We combined two-photon fluorescence lifetime imaging with two-photon glutamate uncaging to image the activity of the small guanosine triphosphatase Ras after NMDA receptor activation at individual spines. Induction of long-term potentiation (LTP) triggered robust Ca2+-dependent Ras activation in single spines that decayed in ∼5 minutes. Ras activity spread over ∼10 micrometers of dendrite and invaded neighboring spines by diffusion. The spread of Ras-dependent signaling was necessary for the local regulation of the threshold for LTP induction. Thus, Ca2+-dependent synaptic signals can spread to couple multiple synapses on short stretches of dendrite.",

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T1 - The spread of Ras activity triggered by activation of a single dendritic spine

AU - Harvey, Christopher D.

AU - Yasuda, Ryohei

AU - Zhong, Haining

AU - Svoboda, Karel

PY - 2008/7/4

Y1 - 2008/7/4

N2 - In neurons, individual dendritic spines isolate N-methyl-D-aspartate (NMDA) receptor-mediated calcium ion (Ca2+) accumulations from the dendrite and other spines. However, the extent to which spines compartmentalize signaling events downstream of Ca2+ influx is not known. We combined two-photon fluorescence lifetime imaging with two-photon glutamate uncaging to image the activity of the small guanosine triphosphatase Ras after NMDA receptor activation at individual spines. Induction of long-term potentiation (LTP) triggered robust Ca2+-dependent Ras activation in single spines that decayed in ∼5 minutes. Ras activity spread over ∼10 micrometers of dendrite and invaded neighboring spines by diffusion. The spread of Ras-dependent signaling was necessary for the local regulation of the threshold for LTP induction. Thus, Ca2+-dependent synaptic signals can spread to couple multiple synapses on short stretches of dendrite.

AB - In neurons, individual dendritic spines isolate N-methyl-D-aspartate (NMDA) receptor-mediated calcium ion (Ca2+) accumulations from the dendrite and other spines. However, the extent to which spines compartmentalize signaling events downstream of Ca2+ influx is not known. We combined two-photon fluorescence lifetime imaging with two-photon glutamate uncaging to image the activity of the small guanosine triphosphatase Ras after NMDA receptor activation at individual spines. Induction of long-term potentiation (LTP) triggered robust Ca2+-dependent Ras activation in single spines that decayed in ∼5 minutes. Ras activity spread over ∼10 micrometers of dendrite and invaded neighboring spines by diffusion. The spread of Ras-dependent signaling was necessary for the local regulation of the threshold for LTP induction. Thus, Ca2+-dependent synaptic signals can spread to couple multiple synapses on short stretches of dendrite.

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The spread of Ras activity triggered by activation of a single dendritic spine

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