Abstract
A rapid technique for separating and quantitating the three enolase isozymes present in rodent brain and sciatic nerve was developed using high-pressure liquid anion-exchange chromatography. At pH 7.9, one cationic and two anionic enzyme forms were separated with baseline resolution in an imidazole buffer containing ethylenediaminetetraacetic acid (EDTA) and magnesium. The recovery of enolase activity was 90% or greater for brain and 85% for sciatic nerve. Chromatography of liver and axon-free (degenerated) sciatic nerve allowed the identification of non-neuronal, hybrid, and neuron-specific enolase isozymes. These enzyme forms, respectively, constituted 40%, 29% and 19% of total activity in brain, and 63%, 13% and 4% of total activity in normal sciatic nerve.
Original language | English (US) |
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Pages (from-to) | 196-199 |
Number of pages | 4 |
Journal | Brain research |
Volume | 342 |
Issue number | 1 |
DOIs | |
State | Published - Sep 2 1985 |
Externally published | Yes |
Keywords
- high-pressure liquid chromatography
- neuron-specific enolase
- rodent brain and sciatic nerve
ASJC Scopus subject areas
- General Neuroscience
- Molecular Biology
- Clinical Neurology
- Developmental Biology