The separation and identification of enolase isozymes of brain and sciatic nerve by high-pressure liquid anion-exchange chromatography

Andrew I. Soiefer, Matthew S. Miller, Mohammad I. Sabri, Peter S. Spencer

Research output: Contribution to journalArticle

Abstract

A rapid technique for separating and quantitating the three enolase isozymes present in rodent brain and sciatic nerve was developed using high-pressure liquid anion-exchange chromatography. At pH 7.9, one cationic and two anionic enzyme forms were separated with baseline resolution in an imidazole buffer containing ethylenediaminetetraacetic acid (EDTA) and magnesium. The recovery of enolase activity was 90% or greater for brain and 85% for sciatic nerve. Chromatography of liver and axon-free (degenerated) sciatic nerve allowed the identification of non-neuronal, hybrid, and neuron-specific enolase isozymes. These enzyme forms, respectively, constituted 40%, 29% and 19% of total activity in brain, and 63%, 13% and 4% of total activity in normal sciatic nerve.

Original languageEnglish (US)
Pages (from-to)196-199
Number of pages4
JournalBrain research
Volume342
Issue number1
DOIs
StatePublished - Sep 2 1985

Keywords

  • high-pressure liquid chromatography
  • neuron-specific enolase
  • rodent brain and sciatic nerve

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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