The role of cytochrome P450 2C3 in rabbit liver microsomal metabolism of 1‐nitropyrene and 3‐nitrofluoranthene

Paul C. Howard, Michael E. McManus, Dennis R. Koop

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The male rabbit liver microsomal cytochrome P450 metabolism of 1‐nitropyrene and 3‐nitrofluoranthene was investigated. In this study, we used inhibitory antibodies specific for rabbit P450 2C3 and determined that, in untreated male rabbit liver microsomes, the antibody inhibited approximately 75% of the cytochrome P450‐mediated C‐oxidation of both 1‐nitropyrene and 3‐nitrofluoranthene. These results verify our previous prediction that mainly one cytochrome P450 is responsible for microsomal metabolism of 1‐nitropyrene in the untreated rabbit liver.

Original languageEnglish (US)
Pages (from-to)71-78
Number of pages8
JournalJournal of Biochemical Toxicology
Volume9
Issue number2
DOIs
StatePublished - Apr 1994

Keywords

  • 1‐Nitropyrene
  • 3‐Nitrofluoranthene
  • Cytochrome P450
  • Environmental Carcinogen
  • Microsomal Metabolism

ASJC Scopus subject areas

  • Toxicology

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