The role of calcitonin gene-related peptide in cutaneous immunosuppression induced by repeated subinflammatory ultraviolet irradiation exposure

Franz J. Legat, Lasha T. Jaiani, Peter Wolf, Minsheng Wang, Roland Lang, Tonya Abraham, Alvin Jr Solomon, Cheryl A. Armstrong, Jonathan D. Glass, John C. Ansel

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Ultraviolet (UV) light is an effective treatment for skin disorders like psoriasis in which the cutaneous neurosensory system may have a pathogenic role. In this study, we examined the possibility that UV modulation of the cutaneous neurosensory system and calcitonin gene-related peptide (CGRP) may contribute to local immunosuppression mediated by repeated subinflammatory UV irradiation. Our results indicated that exposure of hairless mice to subinflammatory UV three times weekly for 4 weeks significantly increased the number of epidermal nerve fibers (ENFs) immunoreactive for CGRP without altering the total number of ENFs. The skin content of CGRP as measured by enzyme-linked immunosorbent assay was also significantly increased after exposure to this dose of UV. These effects were most apparent 1 day after the last UV exposure and declined 1 week after UV. The role of CGRP in UV-induced immunosuppression of contact hypersensitivity was then examined. Our results indicated that UV suppression of epicutaneous 2,4-dinitro-1-fluorobenzene (DNFB) sensitization could be significantly inhibited by a systemically administered CGRP receptor antagonist. A broad-spectrum sunscreen applied before UV exposure inhibited increased cutaneous CGRP and blocked immunosuppression. These findings support a role for CGRP in the local immunosuppression caused by chronic, repeated subinflammatory UV exposure.

Original languageEnglish (US)
Pages (from-to)242-250
Number of pages9
JournalExperimental Dermatology
Volume13
Issue number4
DOIs
StatePublished - Apr 2004
Externally publishedYes

Fingerprint

Calcitonin Gene-Related Peptide
Immunosuppression
Irradiation
Skin
Nerve Fibers
Fluorobenzenes
Calcitonin Gene-Related Peptide Receptors
Sun hoods
Hairless Mouse
Sunscreening Agents
Immunosorbents
Fibers
Contact Dermatitis
Ultraviolet Rays
Psoriasis
Assays
Enzyme-Linked Immunosorbent Assay
Modulation
Enzymes

Keywords

  • Broad-spectrum sunscreen
  • CGRP
  • Contact hypersensitivity reaction
  • Epidermal nerve fibers
  • Neuropeptides

ASJC Scopus subject areas

  • Dermatology

Cite this

The role of calcitonin gene-related peptide in cutaneous immunosuppression induced by repeated subinflammatory ultraviolet irradiation exposure. / Legat, Franz J.; Jaiani, Lasha T.; Wolf, Peter; Wang, Minsheng; Lang, Roland; Abraham, Tonya; Solomon, Alvin Jr; Armstrong, Cheryl A.; Glass, Jonathan D.; Ansel, John C.

In: Experimental Dermatology, Vol. 13, No. 4, 04.2004, p. 242-250.

Research output: Contribution to journalArticle

Legat, FJ, Jaiani, LT, Wolf, P, Wang, M, Lang, R, Abraham, T, Solomon, AJ, Armstrong, CA, Glass, JD & Ansel, JC 2004, 'The role of calcitonin gene-related peptide in cutaneous immunosuppression induced by repeated subinflammatory ultraviolet irradiation exposure', Experimental Dermatology, vol. 13, no. 4, pp. 242-250. https://doi.org/10.1111/j.0906-6705.2004.00185.x
Legat, Franz J. ; Jaiani, Lasha T. ; Wolf, Peter ; Wang, Minsheng ; Lang, Roland ; Abraham, Tonya ; Solomon, Alvin Jr ; Armstrong, Cheryl A. ; Glass, Jonathan D. ; Ansel, John C. / The role of calcitonin gene-related peptide in cutaneous immunosuppression induced by repeated subinflammatory ultraviolet irradiation exposure. In: Experimental Dermatology. 2004 ; Vol. 13, No. 4. pp. 242-250.
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