The relationship between dose of vitamin E and suppression of oxidative stress in humans

L. Jackson Roberts, John A. Oates, MacRae F. Linton, Sergio Fazio, Beth P. Meador, Myron D. Gross, Yu Shyr, Jason D. Morrow

Research output: Contribution to journalArticle

171 Citations (Scopus)

Abstract

The oxidation hypothesis of atherogenesis has been the focus of much research over the past 2 decades. However, randomized placebo-controlled trials evaluating the efficacy of vitamin E in preventing cardiovascular events in aggregate have failed to show a beneficial effect. Implicit in these trials is that the dose of vitamin E tested effectively suppressed oxidative stress status but this was never determined. We defined the dose-dependent effects of vitamin E (RRR-α-tocopherol) to suppress plasma concentrations of F2-isoprostanes, a biomarker of free radical-mediated lipid peroxidation, in participants with polygenic hypercholesterolemia and enhanced oxidative stress, a population at risk for cardiovascular events. A time-course study was first performed in participants supplemented with 3200 IU/day of vitamin E for 20 weeks. A dose-ranging study was then performed in participants supplemented with 0, 100, 200, 400, 800, 1600, or 3200 IU/day of vitamin E for 16 weeks. In the time-course study, maximum suppression of plasma F2-isoprostane concentrations did not occur until 16 weeks of supplementation. In the dose-ranging study there was a linear trend between the dosage of vitamin E and percentage reduction in plasma F2-isoprostane concentrations which reached significance at doses of 1600 IU (35 ± 2%, p <0.035) and 3200 IU (49 ± 10%, p <0.005). This study provides information on the dosage of vitamin E that decreases systemic oxidant stress in vivo in humans and informs the planning and evaluation of clinical studies that assess the efficacy of vitamin E to mitigate disease.

Original languageEnglish (US)
Pages (from-to)1388-1393
Number of pages6
JournalFree Radical Biology and Medicine
Volume43
Issue number10
DOIs
StatePublished - Nov 15 2007
Externally publishedYes

Fingerprint

Oxidative stress
Vitamin E
Oxidative Stress
F2-Isoprostanes
Time and motion study
Plasmas
Tocopherols
Biomarkers
Hypercholesterolemia
Oxidants
Lipid Peroxidation
Free Radicals
Atherosclerosis
Randomized Controlled Trials
Placebos
Lipids
Planning
Oxidation
Research

Keywords

  • Cardiovascular disease
  • Free radicals
  • Hypercholesterolemia
  • Isoprostane
  • Oxidative stress
  • Vitamin E

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

Roberts, L. J., Oates, J. A., Linton, M. F., Fazio, S., Meador, B. P., Gross, M. D., ... Morrow, J. D. (2007). The relationship between dose of vitamin E and suppression of oxidative stress in humans. Free Radical Biology and Medicine, 43(10), 1388-1393. https://doi.org/10.1016/j.freeradbiomed.2007.06.019

The relationship between dose of vitamin E and suppression of oxidative stress in humans. / Roberts, L. Jackson; Oates, John A.; Linton, MacRae F.; Fazio, Sergio; Meador, Beth P.; Gross, Myron D.; Shyr, Yu; Morrow, Jason D.

In: Free Radical Biology and Medicine, Vol. 43, No. 10, 15.11.2007, p. 1388-1393.

Research output: Contribution to journalArticle

Roberts, LJ, Oates, JA, Linton, MF, Fazio, S, Meador, BP, Gross, MD, Shyr, Y & Morrow, JD 2007, 'The relationship between dose of vitamin E and suppression of oxidative stress in humans', Free Radical Biology and Medicine, vol. 43, no. 10, pp. 1388-1393. https://doi.org/10.1016/j.freeradbiomed.2007.06.019
Roberts, L. Jackson ; Oates, John A. ; Linton, MacRae F. ; Fazio, Sergio ; Meador, Beth P. ; Gross, Myron D. ; Shyr, Yu ; Morrow, Jason D. / The relationship between dose of vitamin E and suppression of oxidative stress in humans. In: Free Radical Biology and Medicine. 2007 ; Vol. 43, No. 10. pp. 1388-1393.
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