The PKA-C3 catalytic subunit is required in two pairs of interneurons for successful mating of Drosophila

Marlène Cassar, Elizabeth Sunderhaus, Jill S. Wentzell, Sara Kuntz, Roland Strauss, Doris Kretzschmar

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Protein kinase A (PKA) has been shown to play a role in a plethora of cellular processes ranging from development to memory formation. Its activity is mediated by the catalytic subunits whereby many species express several paralogs. Drosophila encodes three catalytic subunits (PKA-C1-3) and whereas PKA-C1 has been well studied, the functions of the other two subunits were unknown. PKA-C3 is the orthologue of mammalian PRKX/Pkare and they are structurally more closely related to each other than to other catalytic subunits within their species. PRKX is expressed in the nervous system in mice but its function is also unknown. We now show that the loss of PKA-C3 in Drosophila causes copulation defects, though the flies are active and show no defects in other courtship behaviours. This phenotype is specifically due to the loss of PKA-C3 because PKA-C1 cannot replace PKA-C3. PKA-C3 is expressed in two pairs of interneurons that send projections to the ventro-lateral protocerebrum and the mushroom bodies and that synapse onto motor neurons in the ventral nerve cord. Rescue experiments show that expression of PKA-C3 in these interneurons is sufficient for copulation, suggesting a role in relaying information from the sensory system to motor neurons to initiate copulation.

Original languageEnglish (US)
Article number2458
JournalScientific Reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

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Interneurons
Cyclic AMP-Dependent Protein Kinases
Drosophila
Catalytic Domain
Copulation
Motor Neurons
Cyclic AMP-Dependent Protein Kinase Catalytic Subunits
Mushroom Bodies
Courtship
Information Systems
Diptera
Synapses
Nervous System
Phenotype

ASJC Scopus subject areas

  • General

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The PKA-C3 catalytic subunit is required in two pairs of interneurons for successful mating of Drosophila. / Cassar, Marlène; Sunderhaus, Elizabeth; Wentzell, Jill S.; Kuntz, Sara; Strauss, Roland; Kretzschmar, Doris.

In: Scientific Reports, Vol. 8, No. 1, 2458, 01.12.2018.

Research output: Contribution to journalArticle

Cassar, Marlène ; Sunderhaus, Elizabeth ; Wentzell, Jill S. ; Kuntz, Sara ; Strauss, Roland ; Kretzschmar, Doris. / The PKA-C3 catalytic subunit is required in two pairs of interneurons for successful mating of Drosophila. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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