The phosphoinositide phosphatase Sac1p controls trafficking of the yeast Chs3p chitin synthase

Markus Schorr, Angela Then, Sabina Tahirovic, Nele Hug, Peter Mayinger

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Phosphoinositide phosphatases play an essential but as yet not well-understood role in lipid-based signal transduction. Members of a subfamily of these enzymes share a specific domain that was first identified in the yeast Sac1 protein [1]. Sac1 homology domains were shown to exhibit 3- and 4-phosphatase activity in vitro [2, 3] and were also found, in addition to rat and yeast Sac1p, in yeast Inp/Sjl proteins [4, 5] and mammalian synaptojanins [6]. Despite the detailed in vitro characterization of the enzymatic properties of yeast Sac1p, the exact cellular function of this protein has remained obscure. We report here that Sac1p has a specific role in secretion and acts as an antagonist of the phosphatidylinositol 4-kinase Pik1p in Golgi trafficking. Elimination of Sac1p leads to excessive forward transport of chitin synthases and thus causes specific cell wall defects. Similar defects in membrane trafficking are caused by the overexpression of PIK1. Taken together, these findings provide strong evidence that the generation of Ptdlns(4)P is sufficient to trigger forward transport from the Golgi to the plasma membrane and that Sac1p is critically required for the termination of this signal.

Original languageEnglish (US)
Pages (from-to)1421-1426
Number of pages6
JournalCurrent Biology
Volume11
Issue number18
DOIs
StatePublished - Sep 18 2001
Externally publishedYes

Fingerprint

Chitin Synthase
chitin synthase
Phosphatidylinositols
Phosphoric Monoester Hydrolases
Yeast
Yeasts
yeasts
Signal transduction
Defects
1-Phosphatidylinositol 4-Kinase
Fungal Proteins
1-phosphatidylinositol 4-kinase
Cell membranes
Cell Wall
proteins
Rats
Signal Transduction
Proteins
sequence homology
Cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

The phosphoinositide phosphatase Sac1p controls trafficking of the yeast Chs3p chitin synthase. / Schorr, Markus; Then, Angela; Tahirovic, Sabina; Hug, Nele; Mayinger, Peter.

In: Current Biology, Vol. 11, No. 18, 18.09.2001, p. 1421-1426.

Research output: Contribution to journalArticle

Schorr, Markus ; Then, Angela ; Tahirovic, Sabina ; Hug, Nele ; Mayinger, Peter. / The phosphoinositide phosphatase Sac1p controls trafficking of the yeast Chs3p chitin synthase. In: Current Biology. 2001 ; Vol. 11, No. 18. pp. 1421-1426.
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