The native T-type calcium current in relay neurons of the primate thalamus

G. M. Alexander, W. B. Carden, J. Mu, N. C. Kurukulasuriya, B. A. Mccool, B. K. Nordskog, D. P. Friedman, J. B. Daunais, Kathleen (Kathy) Grant, D. W. Godwin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The generation of thalamic bursts depends upon calcium currents that flow through transiently open (T)-type calcium channels. In this study, we characterized the native T-type calcium current underlying thalamic burst responses in the macaque monkey. Current clamp recordings from lateral geniculate nucleus (LGN) slices showed characteristic burst responses when relay cells were depolarized from relatively hyperpolarized membrane potentials. These bursts could also be elicited by stimulation of excitatory synaptic inputs to LGN cells. Under voltage clamp conditions, the inactivation kinetics of native currents recorded from primate LGN neurons showed consistency with T-type currents recorded in other mammals and in expression systems. Realtime reverse transcriptase PCR performed on RNA isolated from the LGN (including tissues isolated from magnocellular and parvocellular laminae) detected voltage-dependent calcium channel (Cav) 3.1, Cav 3.2, and Cav 3.3 channel transcripts. Cav 3.1 occurred at relatively higher expression than other isoforms, consistent with in situ hybridization studies in rats, indicating that the molecular basis for burst firing in thalamocortical systems is an important conserved property of primate physiology. Since thalamic bursts have been observed during visual processing as well as in a number of CNS disorders, studies of the expression and modulation of these currents at multiple levels are critical for understanding their role in vision and for the discovery of new treatments for disruptions of thalamic rhythms.

Original languageEnglish (US)
Pages (from-to)453-461
Number of pages9
JournalNeuroscience
Volume141
Issue number1
DOIs
StatePublished - 2006
Externally publishedYes

Fingerprint

Geniculate Bodies
Thalamus
Primates
Calcium
Neurons
T-Type Calcium Channels
Macaca
Calcium Channels
Reverse Transcriptase Polymerase Chain Reaction
Membrane Potentials
Haplorhini
In Situ Hybridization
Mammals
Protein Isoforms
RNA

Keywords

  • Burst
  • Cynomolgus macaque
  • Epilepsy
  • EPSP
  • Lateral geniculate nucleus
  • Monkey

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Alexander, G. M., Carden, W. B., Mu, J., Kurukulasuriya, N. C., Mccool, B. A., Nordskog, B. K., ... Godwin, D. W. (2006). The native T-type calcium current in relay neurons of the primate thalamus. Neuroscience, 141(1), 453-461. https://doi.org/10.1016/j.neuroscience.2006.03.042

The native T-type calcium current in relay neurons of the primate thalamus. / Alexander, G. M.; Carden, W. B.; Mu, J.; Kurukulasuriya, N. C.; Mccool, B. A.; Nordskog, B. K.; Friedman, D. P.; Daunais, J. B.; Grant, Kathleen (Kathy); Godwin, D. W.

In: Neuroscience, Vol. 141, No. 1, 2006, p. 453-461.

Research output: Contribution to journalArticle

Alexander, GM, Carden, WB, Mu, J, Kurukulasuriya, NC, Mccool, BA, Nordskog, BK, Friedman, DP, Daunais, JB, Grant, KK & Godwin, DW 2006, 'The native T-type calcium current in relay neurons of the primate thalamus', Neuroscience, vol. 141, no. 1, pp. 453-461. https://doi.org/10.1016/j.neuroscience.2006.03.042
Alexander GM, Carden WB, Mu J, Kurukulasuriya NC, Mccool BA, Nordskog BK et al. The native T-type calcium current in relay neurons of the primate thalamus. Neuroscience. 2006;141(1):453-461. https://doi.org/10.1016/j.neuroscience.2006.03.042
Alexander, G. M. ; Carden, W. B. ; Mu, J. ; Kurukulasuriya, N. C. ; Mccool, B. A. ; Nordskog, B. K. ; Friedman, D. P. ; Daunais, J. B. ; Grant, Kathleen (Kathy) ; Godwin, D. W. / The native T-type calcium current in relay neurons of the primate thalamus. In: Neuroscience. 2006 ; Vol. 141, No. 1. pp. 453-461.
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