The Molecular Basis for Ubiquitin and Ubiquitin-like Specificities in Bacterial Effector Proteases

Jonathan Pruneda, Charlotte H. Durkin, Paul P. Geurink, Huib Ovaa, Balaji Santhanam, David W. Holden, David Komander

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Pathogenic bacteria rely on secreted effector proteins to manipulate host signaling pathways, often in creative ways. CE clan proteases, specific hydrolases for ubiquitin-like modifications (SUMO and NEDD8) in eukaryotes, reportedly serve as bacterial effector proteins with deSUMOylase, deubiquitinase, or, even, acetyltransferase activities. Here, we characterize bacterial CE protease activities, revealing K63-linkage-specific deubiquitinases in human pathogens, such as Salmonella, Escherichia, and Shigella, as well as ubiquitin/ubiquitin-like cross-reactive enzymes in Chlamydia, Rickettsia, and Xanthomonas. Five crystal structures, including ubiquitin/ubiquitin-like complexes, explain substrate specificities and redefine relationships across the CE clan. Importantly, this work identifies novel family members and provides key discoveries among previously reported effectors, such as the unexpected deubiquitinase activity in Xanthomonas XopD, contributed by an unstructured ubiquitin binding region. Furthermore, accessory domains regulate properties such as subcellular localization, as exemplified by a ubiquitin-binding domain in Salmonella Typhimurium SseL. Our work both highlights and explains the functional adaptations observed among diverse CE clan proteins.

Original languageEnglish (US)
Pages (from-to)261-276
Number of pages16
JournalMolecular Cell
Volume63
Issue number2
DOIs
StatePublished - Jul 21 2016
Externally publishedYes

Fingerprint

Ubiquitin
Peptide Hydrolases
Xanthomonas
Ubiquitin-Specific Proteases
Escherichia
Rickettsia
Acetyltransferases
Shigella
Bacterial Proteins
Chlamydia
Hydrolases
Salmonella typhimurium
Substrate Specificity
Eukaryota
Salmonella
Proteins
Bacteria
Enzymes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Pruneda, J., Durkin, C. H., Geurink, P. P., Ovaa, H., Santhanam, B., Holden, D. W., & Komander, D. (2016). The Molecular Basis for Ubiquitin and Ubiquitin-like Specificities in Bacterial Effector Proteases. Molecular Cell, 63(2), 261-276. https://doi.org/10.1016/j.molcel.2016.06.015

The Molecular Basis for Ubiquitin and Ubiquitin-like Specificities in Bacterial Effector Proteases. / Pruneda, Jonathan; Durkin, Charlotte H.; Geurink, Paul P.; Ovaa, Huib; Santhanam, Balaji; Holden, David W.; Komander, David.

In: Molecular Cell, Vol. 63, No. 2, 21.07.2016, p. 261-276.

Research output: Contribution to journalArticle

Pruneda, J, Durkin, CH, Geurink, PP, Ovaa, H, Santhanam, B, Holden, DW & Komander, D 2016, 'The Molecular Basis for Ubiquitin and Ubiquitin-like Specificities in Bacterial Effector Proteases', Molecular Cell, vol. 63, no. 2, pp. 261-276. https://doi.org/10.1016/j.molcel.2016.06.015
Pruneda, Jonathan ; Durkin, Charlotte H. ; Geurink, Paul P. ; Ovaa, Huib ; Santhanam, Balaji ; Holden, David W. ; Komander, David. / The Molecular Basis for Ubiquitin and Ubiquitin-like Specificities in Bacterial Effector Proteases. In: Molecular Cell. 2016 ; Vol. 63, No. 2. pp. 261-276.
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