The influence of antiadhesion therapies on leukocyte subset accumulation in central nervous system ischemia in rats

Wayne Clark, Jeff D. Lauten, Nikola Lessov, William Woodward, Bruce M. Coull

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Although treatment with agents that block leukocyte function, including anti-ICAM-1 and doxycycline, reduces experimental central nervous system (CNS) ischemic injury, it is not known how leukocyte subset accumulation is affected by these agents. Using the rat two-vessel occlusion model and immunohistochemistry, we investigated granulocyte (PMN) and monocyte/macrophage (Mφ) accumulation at 1 and 4 d postischemia. A total of 24 animals were randomized to sham surgery, or to ischemia with saline, anti-ICAM-1, or doxycycline treatments. No leukocytes were observed in sham animals. At 24 h postischemia, there was a moderate infiltration of PMN and Mφ in untreated animals that was significantly decreased with either treatment. At 4 d after ischemia no PMN were identified, with extensive Mφ accumulation occurring in untreated animals that was only partially reduced with doxycycline treatment. These results confirm that both anti-ICAM-1 and doxycycline treatments reduce PMN and Mφ infiltration at 24 h. Delayed Mφ accumulation occurs despite treatment, suggesting that some of these cells represent transformed resident microglia.

Original languageEnglish (US)
Pages (from-to)43-50
Number of pages8
JournalJournal of Molecular Neuroscience
Volume6
Issue number1
DOIs
StatePublished - Mar 1995

Fingerprint

Doxycycline
Neurology
Rats
Animals
Leukocytes
Intercellular Adhesion Molecule-1
Ischemia
Central Nervous System
Infiltration
Macrophages
Therapeutics
Surgery
Nervous System Trauma
Microglia
Granulocytes
Monocytes
Immunohistochemistry

Keywords

  • Cerebral ischemia
  • doxycycline
  • granulocytes
  • ICAM-1
  • macrophages

ASJC Scopus subject areas

  • Neuroscience(all)
  • Genetics
  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

The influence of antiadhesion therapies on leukocyte subset accumulation in central nervous system ischemia in rats. / Clark, Wayne; Lauten, Jeff D.; Lessov, Nikola; Woodward, William; Coull, Bruce M.

In: Journal of Molecular Neuroscience, Vol. 6, No. 1, 03.1995, p. 43-50.

Research output: Contribution to journalArticle

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