The human dopamine D2 receptor gene is located on Chromosome 11 at q22-q23 and identifies a TaqI RFLP

D. K. Grandy, M. Litt, L. Allen, J. R. Bunzow, M. Marchionni, H. Makam, L. Reed, R. E. Magenis, O. Civelli

    Research output: Contribution to journalArticlepeer-review

    376 Scopus citations

    Abstract

    Human dopaminergic neurons are involved in the control of hormone secretion, voluntary movement, and emotional behavior. Mediating these effects are the dopamine D1 and D2 receptors. These macromolecules belong to a large family of related sequences known as the G protein-coupled receptors. The D2 receptors have been of special interest because they bind, with high affinity and specificity, many of the commonly prescribed antipsychotic drugs. We previously isolated a full-length cDNA clone of the rat D2 receptor. When a chromosome mapping panel was probed with the rat D2 receptor cDNA a 15-kb EcoRI restriction fragment was identified and localized to human chromosome 11. The rat cDNA was also used to clone a human genomic fragment, λhD2G1, which contains the last coding exon of the D2 receptor gene (DRD2) and 16.5 kb of 3' flanking sequence. Hybridization of λhD2G1 to a chromosome 11 regional mapping panel localized DRD2 to 11q. In situ hybridization of λhD2G1 to metaphase chromosomes refined this asignment to the q22-q23 junction of chromosome 11. A search for RFLPs associated with D2DR identified a frequent two-allele TaqI RFLP.

    Original languageEnglish (US)
    Pages (from-to)778-785
    Number of pages8
    JournalAmerican Journal of Human Genetics
    Volume45
    Issue number5
    StatePublished - Jan 1 1989

    ASJC Scopus subject areas

    • Genetics
    • Genetics(clinical)

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