The hSNM1 protein is a DNA 5′-exonuclease

James Hejna, Sahaayaruban Philip, Jesse Ott, Craig Faulkner, Robb Moses

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

The human SNM1 protein is a member of a highly conserved group of proteins catalyzing the hydrolysis of nucleic acid substrates. Although overproduction is unstable in mammalian cells, we have overproduced a recombinant hSNM1 protein in an insect cell system. The protein is a single-strand 5′-exonuclease, like its yeast homolog. The enzyme utilizes either DNA or RNA substrates, requires a 5′-phosphate moiety, shows very little activity on double-strand substrates, and functions at a size consistent with a monomer. The exonuclease activity requires the conserved β-lactamase domain; site-directed mutagenesis of a conserved aspartate inactivates the exonuclease.

Original languageEnglish (US)
Pages (from-to)6115-6123
Number of pages9
JournalNucleic acids research
Volume35
Issue number18
DOIs
StatePublished - Sep 2007

ASJC Scopus subject areas

  • Genetics

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